Expression of HLA‐C‐specific natural killer cell receptors (CD158a and CD158b) on peripheral blood mononuclear cells after allogeneic bone marrow transplantation
Expression of HLA‐C‐specific natural killer cell receptors (CD158a and CD158b) on peripheral blood mononuclear cells after allogeneic bone marrow transplantation
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同种异体骨髓移植后外周血单个核细胞上HLA-C特异性自然杀伤细胞受体（CD158a和CD158b）的表达
DOI：
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发表时间：
2000
影响因子：
6.5
通讯作者：
M. Imamura
中科院分区：
文献类型：
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作者：
Junji Tanaka;A. Mori;Shuichi Ohta;Yasushi Yamamoto;Sumiko Kobayashi;S. Hashino;Masanobu Kobayashi;M. Asaka;M. Imamura
我们研究了23例异基因骨髓移植（allo-BMT）患者外周血单个核细胞（PBMC）上HLA-C的自然杀伤细胞受体（NKRs）的表达，以分析NKRs在移植物抗宿主病（GVHD）同种异体反应中的作用。CD 158 a表达较低，同种异体BMT后表达变化不大。此外，异基因骨髓移植后CD 158 a +/CD 3 −的比例没有差异。相比之下，CD 158 b +/CD 3 −细胞（主要是NK细胞）的比例在异基因骨髓移植后早期（< 2个月）增加，然后逐渐下降（骨髓移植前3.3±2.6% vs.骨髓移植后早期15.4±8.6%，骨髓移植后3-6个月8.5±4.9%，骨髓移植后6个月7.0± 3.0%; P<0.05）。 然而，异基因骨髓移植后3个月，CD 3 + T细胞上的CD 158 b表达增加（骨髓移植前为1.1±1.1%，骨髓移植后3-6个月为5.1±7.7%，骨髓移植后6个月为3.0± 2.4%，P<0.05）。 比较了无慢性GVHD（cGVHD）患者和有cGVHD患者中CD 158表达的最高百分比。cGVHD患者外周血CD 158 b +/CD 3+和CD 158 b +/CD 8+细胞百分率分别为2.6± 2.0%和2.3 ± 1.5%，明显高于非cGVHD患者（8.0 ± 11.2%和8.3 ±11.7%，P <0.05）。 这些表达CD 158 b的细胞的确切临床相关性尚不清楚。然而，有一种有趣的可能性，即表达CD 158 b的细胞在allo-BMT后GVHD的调节中发挥一定作用。
We investigated the expression of natural killer cell receptors (NKRs) for HLA‐C on peripheral blood mononuclear cells (PBMCs) in 23 allogeneic bone marrow transplantation (allo‐BMT) patients to analyse the role of NKRs in alloresponse concerning graft‐versus‐host disease (GVHD). CD158a expression was low and there was little change in the expression after allo‐BMT. Also, there was no difference in the proportion of CD158a+/CD3− after allo‐BMT. In contrast, the proportion of CD158b+/CD3− cells, mainly NK cells, increased in the early stage (< 2 months) after allo‐BMT and then gradually decreased (3.3 ± 2.6% before BMT vs. 15.4 ± 8.6% in the early stage after BMT, 8.5 ± 4.9% during the period 3–6 months after BMT and 7.0 ± 3.0% > 6 months after BMT; P < 0.05). However, CD158b expression on CD3+ T cells increased 3 months after allo‐BMT (1.1 ± 1.1% before BMT vs. 5.1 ± 7.7% during the period 3–6 months after BMT and 3.0 ± 2.4% > 6 months after BMT, P < 0.05). The highest percentages of CD158 expression in patients without chronic GVHD (cGVHD) and those with cGVHD were compared. The percentage of CD158b+/CD3+ cells and also that of CD158b+/CD8+ cells were significantly increased in patients with cGVHD compared with those in patients without cGVHD (2.6 ± 2.0% vs. 8.0 ± 11.2% and 2.3 ± 1.5% vs. 8.3 ± 11.7% respectively; P < 0.05). The exact clinical relevance of these CD158b‐expressing cells is not clear. However, there is an interesting possibility that CD158b‐expressing cells play some role in the regulation of GVHD after allo‐BMT.
NKB1 和 HP-3E4 NK 细胞受体是结构不同的糖蛋白，独立识别多态性 HLA-B 和 HLA-C 分子。
DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lanier,LL;Gumperz,JE;Parham,P;Melero,I;Lopez-Botet,M;Phillips,JH
通讯作者：
Phillips,JH
影响因子：
32.4
作者：
Valiante, NM;Uhrberg, M;Parham, P
通讯作者：
Parham, P
影响因子：
56.9
作者：
PHILLIPS, JH;GUMPERZ, JE;LANIER, LL
通讯作者：
LANIER, LL