Modular prophage interactions driven by capsule serotype select for capsule loss under phage predation.
Modular prophage interactions driven by capsule serotype select for capsule loss under phage predation.
复制标题
DOI：
10.1038/s41396-020-0726-z
复制
发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Rendueles O
中科院分区：
文献类型：
--
作者：
de Sousa JAM;Buffet A;Haudiquet M;Rocha EPC;Rendueles O
克雷伯氏菌能够在广泛的环境中定居，包括令人担忧的医院病原体。在这里，我们试图确定克雷伯氏菌前驱体的丰度和传染性，以了解诱导前驱体和细菌之间的相互作用如何影响种群动态和进化。我们在该物种中发现了许多预言体，将这些分类群置于最具多解性细菌的前5%之列。我们选择了35株肺炎克雷伯菌菌种复合体的代表性菌株来建立一个诱导噬菌体-细菌相互作用的网络。这表明许多原噬菌体能够进入裂解周期，随后杀死或裂解密切相关的克雷伯氏菌菌株。虽然60%的测试菌株可以产生至少感染一种其他菌株的噬菌体，但所有成对交叉感染的相互作用网络非常稀疏，大多组织在与菌株的被膜血清型相对应的模块中。因此，衣壳突变体仍未被感染，这表明衣壳是成功感染的关键因素。令人惊讶的是，细菌被它们自己的预言体先于细菌的实验结果是胶囊加速丢失。我们的结果表明，噬菌体感染性定义了小部分噬菌体与细菌之间的相互作用模块，这些模块具有被膜血清型的作用。这限制了前驱体作为竞争性武器的作用，因为它们可以感染物种复合体中的极少数菌株。这也应该限制噬菌体驱动的基因跨物种流动。最后，被自身噬菌体先于细菌的包膜加速丧失，表明噬菌体在自然界中驱动血清型转换。
Klebsiella species are able to colonize a wide range of environments and include worrisome nosocomial pathogens. Here, we sought to determine the abundance and infectivity of prophages of Klebsiella to understand how the interactions between induced prophages and bacteria affect population dynamics and evolution. We identified many prophages in the species, placing these taxa among the top 5% of the most polylysogenic bacteria. We selected 35 representative strains of the Klebsiella pneumoniae species complex to establish a network of induced phage–bacteria interactions. This revealed that many prophages are able to enter the lytic cycle, and subsequently kill or lysogenize closely related Klebsiella strains. Although 60% of the tested strains could produce phages that infect at least one other strain, the interaction network of all pairwise cross-infections is very sparse and mostly organized in modules corresponding to the strains’ capsule serotypes. Accordingly, capsule mutants remain uninfected showing that the capsule is a key factor for successful infections. Surprisingly, experiments in which bacteria are predated by their own prophages result in accelerated loss of the capsule. Our results show that phage infectiousness defines interaction modules between small subsets of phages and bacteria in function of capsule serotype. This limits the role of prophages as competitive weapons because they can infect very few strains of the species complex. This should also restrict phage-driven gene flow across the species. Finally, the accelerated loss of the capsule in bacteria being predated by their own phages, suggests that phages drive serotype switch in nature.
登录
查看更多内容
影响因子：
3.7
作者：
CALEF, E;MARCHELLI, C;GUERRINI, F
通讯作者：
GUERRINI, F
影响因子：
10.7
作者：
Bobay LM;Rocha EP;Touchon M
通讯作者：
Touchon M
影响因子：
6.7
作者：
Asadulghani M;Ogura Y;Ooka T;Itoh T;Sawaguchi A;Iguchi A;Nakayama K;Hayashi T
通讯作者：
Hayashi T
影响因子：
12.3
作者：
Daubin V;Lerat E;Perrière G
通讯作者：
Perrière G
影响因子：
6.4
作者：
Allen HK;Looft T;Bayles DO;Humphrey S;Levine UY;Alt D;Stanton TB
通讯作者：
Stanton TB