The adaptation of temperate bacteriophages to their host genomes.
The adaptation of temperate bacteriophages to their host genomes.
复制标题
DOI：
10.1093/molbev/mss279
复制
发表时间：
2013-04
影响因子：
10.7
通讯作者：
Touchon M
中科院分区：
文献类型：
--
作者：
Bobay LM;Rocha EP;Touchon M
移动的元件的快速周转驱动了细菌基因组的可塑性。整合的噬菌体（原噬菌体）编码宿主适应性性状，并代表了相当大的一部分细菌染色体。我们假设，自然选择形状相对于宿主基因组组织的原噬菌体整合模式。我们通过检测和研究69株大肠杆菌和沙门氏菌的500个前噬菌体来验证这一想法。噬菌体整合酶通常不仅靶向保守基因，而且靶向基因间位置，这表明整合位点的纯化选择。此外，大多数整合热点在两个宿主属之间是保守的。整合位点似乎也选择在大的染色体规模，因为它们是非随机组织的起点-末端轴和宏域结构。的基因的拟单胞菌原噬菌体是系统的共定向与细菌的复制叉，并显示主机高频率的极化FtsK定向极性序列基序所需的染色体分离。matS基序被原噬菌体强烈地避免，表明破坏宏结构域的基序的反选择。这些结果显示了染色体中前噬菌体无缝整合的自然选择如何塑造细菌和噬菌体的进化。首先，整合位点在数百万年来高度保守，有利于温带噬菌体的溶原性而不是溶解周期。第二，原噬菌体的全球分布与染色体结构和基因表达模式密切相关。第三，噬菌体经受对DNA基序的选择，这些DNA基序专门属于细菌染色体中的前噬菌体的生物学。了解原噬菌体遗传适应为水平转移和有组织的细菌基因组的共存提供了新的线索。
Rapid turnover of mobile elements drives the plasticity of bacterial genomes. Integrated bacteriophages (prophages) encode host-adaptive traits and represent a sizable fraction of bacterial chromosomes. We hypothesized that natural selection shapes prophage integration patterns relative to the host genome organization. We tested this idea by detecting and studying 500 prophages of 69 strains of Escherichia and Salmonella. Phage integrases often target not only conserved genes but also intergenic positions, suggesting purifying selection for integration sites. Furthermore, most integration hotspots are conserved between the two host genera. Integration sites seem also selected at the large chromosomal scale, as they are nonrandomly organized in terms of the origin–terminus axis and the macrodomain structure. The genes of lambdoid prophages are systematically co-oriented with the bacterial replication fork and display the host high frequency of polarized FtsK-orienting polar sequences motifs required for chromosome segregation. matS motifs are strongly avoided by prophages suggesting counter selection of motifs disrupting macrodomains. These results show how natural selection for seamless integration of prophages in the chromosome shapes the evolution of the bacterium and the phage. First, integration sites are highly conserved for many millions of years favoring lysogeny over the lytic cycle for temperate phages. Second, the global distribution of prophages is intimately associated with the chromosome structure and the patterns of gene expression. Third, the phage endures selection for DNA motifs that pertain exclusively to the biology of the prophage in the bacterial chromosome. Understanding prophage genetic adaptation sheds new lights on the coexistence of horizontal transfer and organized bacterial genomes.
登录
查看更多内容
DOI：
10.1111/j.1749-6632.1980.tb27987.x
发表时间：
1980-01-01
影响因子：
5.2
作者：
Botstein, D
通讯作者：
Botstein, D
影响因子：
3.7
作者：
BUKHARI, AI;METLAY, M
通讯作者：
METLAY, M
影响因子：
7.7
作者：
Cui, Tailin;Moro-oka, Naoki;Horiuchi, Takashi
通讯作者：
Horiuchi, Takashi
影响因子：
6.7
作者：
Asadulghani M;Ogura Y;Ooka T;Itoh T;Sawaguchi A;Iguchi A;Nakayama K;Hayashi T
通讯作者：
Hayashi T
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--