Pan-cancer analysis combined with experiments predicts CTHRC1 as a therapeutic target for human cancers.
Pan-cancer analysis combined with experiments predicts CTHRC1 as a therapeutic target for human cancers.
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泛癌分析结合实验预测CTHRC1作为人类癌症的治疗靶点
DOI：
10.1186/s12935-021-02266-3
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发表时间：
2021-10-26
影响因子：
5.8
通讯作者：
Han L
中科院分区：
文献类型：
--
作者：
Peng D;Wei C;Zhang X;Li S;Liang H;Zheng X;Jiang S;Han L
研究背景含1的胶原三螺旋重复序列(CTHRC1)作为癌基因的功能已被越来越多的文献报道。利用UCSC、NCBI、HPA、TIMER2、Oncomine、GEPIA、UALCAN、cBioPortal、COSMIC、MEXPRESS、STRING、CCLE、LinkedOmics、GTEx、TCGA、CGGA和SangerBox等在线数据库，研究CTHRC1基因与肿瘤发生、发展、甲基化、免疫和预后的关系。结果泛癌分析显示，CTHRC1在大多数肿瘤组织中高表达，且与肿瘤患者的预后密切相关。CTHRC1基因改变发生在多种肿瘤中，并与肿瘤的进展有关。与正常组织相比，大多数癌组织中CTHRC1启动子甲基化水平降低。此外，CTHRC1还通过多种信号转导途径协调ICP基因的活性，并与免疫细胞的侵袭和肿瘤微环境密切相关，是潜在的免疫治疗靶点。我们使用GEPIA2工具确定了癌症中与CTHRC1相关的基因。CTHRC1跨肿瘤的单基因GO分析表明，它参与了一些信号通路和生物学过程，如Wnt信号通路、细胞迁移、蛋白质结合的正向调节等。结论CTHRC1在多种肿瘤类型中均有过表达，是一种重要的癌基因，可能通过不同的机制促进肿瘤的发生发展。CTHRC1可能是治疗人类癌症的一个重要靶点。
BackgroundThe function of collagen triple helix repeat containing 1 (CTHRC1) as an oncogene has been reported in a growing number of publications. Bioinformatics methods represent a beneficial approach to examine the mechanism and function of the CTHRC1 gene in the disease process of cancers from a pan-cancer perspective.MethodsIn this study, using the online databases UCSC, NCBI, HPA, TIMER2, Oncomine, GEPIA, UALCAN, cBioPortal, COSMIC, MEXPRESS, STRING, CCLE, LinkedOmics, GTEx, TCGA, CGGA, and SangerBox, we focused on the relationship between CTHRC1 and tumorigenesis, progression, methylation, immunity, and prognosis. qPCR was used to detect CTHRC1 expression in glioma tissues and cell lines.ResultsThe pan-cancer analysis showed that CTHRC1 was overexpressed in most tumors, and a significant correlation was observed between CTHRC1 expression and the prognosis of patients with cancer. CTHRC1 genetic alterations occur in diverse tumors and are associated with tumor progression. Levels of CTHRC1 promoter methylation were decreased in most cancer tissues compared with normal tissues. In addition, CTHRC1 coordinated the activity of ICP genes through diverse signal transduction pathways, was also associated with immune cell infiltration and the tumor microenvironment, and potentially represented a promising immunotherapy target. We identified CTHRC1-related genes across cancers using the GEPIA2 tool. The single-gene GO analysis of CTHRC1 across cancers showed that it was involved in some signaling pathways and biological processes, such as the Wnt signaling pathway, cell migration, and positive regulation of protein binding. The expression and function of CTHRC1 were also further verified in glioma tissues and cell lines.ConclusionsCTHRC1 is overexpressed in various cancer types and functions as an important oncogene that may promote tumorigenesis and development through different mechanisms. CTHRC1 may represent an important therapeutic target for human cancers.
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
4.4
作者：
Koch A;De Meyer T;Jeschke J;Van Criekinge W
通讯作者：
Van Criekinge W
影响因子：
14.9
作者：
Forbes, Simon A.;Tang, Gurpreet;Futreal, P. Andrew
通讯作者：
Futreal, P. Andrew
影响因子：
12.3
作者：
Li B;Severson E;Pignon JC;Zhao H;Li T;Novak J;Jiang P;Shen H;Aster JC;Rodig S;Signoretti S;Liu JS;Liu XS
通讯作者：
Liu XS
影响因子：
45.3
作者：
Azimi, Farhad;Scolyer, Richard A.;Thompson, John F.
通讯作者：
Thompson, John F.