Human TRIM gene expression in response to interferons.
Human TRIM gene expression in response to interferons.
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DOI：
10.1371/journal.pone.0004894
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Nisole S
中科院分区：
文献类型：
--
作者：
Carthagena L;Bergamaschi A;Luna JM;David A;Uchil PD;Margottin-Goguet F;Mothes W;Hazan U;Transy C;Pancino G;Nisole S
三分基序（TRIM）蛋白是一类具有保守的三分结构的蛋白质家族。最近在灵长类细胞中发现TRIM 5 α具有抗HIV活性，激发了人们对TRIM蛋白在抗病毒活性和先天免疫中的潜在作用的兴趣。为了检测TRIM基因在抗病毒免疫应答期间是否上调，我们对干扰素（IFN，I型和II型）应答或Fcγ R介导的巨噬细胞活化后人原代淋巴细胞和单核细胞衍生的巨噬细胞中的TRIM基因表达进行了系统分析。我们发现72个人类TRIM基因中有27个对IFN敏感。我们的分析确定了9个额外的TRIM基因被IFN上调，其中只有3个先前被发现显示出抗病毒活性。此外，我们发现2个TRIM蛋白，TRIM 9和54，在Fcγ R激活的巨噬细胞中特异性上调。我们的研究结果提出了第一个全面的TRIM基因表达分析在原代人类免疫细胞，并建议参与额外的TRIM蛋白调节宿主的抗病毒活性。
Tripartite motif (TRIM) proteins constitute a family of proteins that share a conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5α in primate cells has stimulated much interest in the potential role of TRIM proteins in antiviral activities and innate immunity. To test if TRIM genes are up-regulated during antiviral immune responses, we performed a systematic analysis of TRIM gene expression in human primary lymphocytes and monocyte-derived macrophages in response to interferons (IFNs, type I and II) or following FcγR-mediated activation of macrophages. We found that 27 of the 72 human TRIM genes are sensitive to IFN. Our analysis identifies 9 additional TRIM genes that are up-regulated by IFNs, among which only 3 have previously been found to display an antiviral activity. Also, we found 2 TRIM proteins, TRIM9 and 54, to be specifically up-regulated in FcγR-activated macrophages. Our results present the first comprehensive TRIM gene expression analysis in primary human immune cells, and suggest the involvement of additional TRIM proteins in regulating host antiviral activities.
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