OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.).
OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.).
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DOI：
10.1186/1471-2229-14-158
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发表时间：
2014-06-06
影响因子：
5.3
通讯作者：
Zhu L
中科院分区：
文献类型：
--
作者：
Yang C;Li D;Liu X;Ji C;Hao L;Zhao X;Li X;Chen C;Cheng Z;Zhu L
草叶形态在农学和发育生物学研究中具有重要的应用价值。卷叶是水稻育种的重要性状之一。MYB转录因子是最大的基因家族之一，在植物发育、代谢和逆境反应中起着重要作用。然而，人们对它们在水稻中的作用知之甚少。在这项研究中，我们报道了水稻基因OsMYB103L的功能特征，该基因编码R2R3-MYB转录因子。OsMYB103L定位于细胞核内，具有活化活性。OsMYB103L在水稻中的过表达导致了卷叶表型。进一步分析表明，OsMYB103L过表达系中几个纤维素合成酶基因（CESAs）的表达量显著增加，纤维素含量也显著增加。通过RNA干扰敲低OsMYB103L导致叶片纤维素含量降低，机械强度降低。同时，在这些敲低系中，多个CESA基因的表达水平降低。这些发现表明，OsMYB103L可能靶向CESA基因调控纤维素合成，并可能通过工程改造使水稻获得理想的叶片形状和机械强度。
The shape of grass leaves possesses great value in both agronomy and developmental biology research. Leaf rolling is one of the important traits in rice (Oryza sativa L.) breeding. MYB transcription factors are one of the largest gene families and have important roles in plant development, metabolism and stress responses. However, little is known about their functions in rice. In this study, we report the functional characterization of a rice gene, OsMYB103L, which encodes an R2R3-MYB transcription factor. OsMYB103L was localized in the nucleus with transactivation activity. Overexpression of OsMYB103L in rice resulted in a rolled leaf phenotype. Further analyses showed that expression levels of several cellulose synthase genes (CESAs) were significantly increased, as was the cellulose content in OsMYB103L overexpressing lines. Knockdown of OsMYB103L by RNA interference led to a decreased level of cellulose content and reduced mechanical strength in leaves. Meanwhile, the expression levels of several CESA genes were decreased in these knockdown lines. These findings suggest that OsMYB103L may target CESA genes for regulation of cellulose synthesis and could potentially be engineered for desirable leaf shape and mechanical strength in rice.
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