Differential Expression of α-Gustducin in Taste Bud Populations of the Rat and Hamster
Differential Expression of α-Gustducin in Taste Bud Populations of the Rat and Hamster
复制标题
α-味导素在大鼠和仓鼠味蕾群体中的差异表达
DOI：
10.1523/jneurosci.17-08-02852.1997
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
David V. Smith
中科院分区：
文献类型：
--
作者：
John D. Boughter Jr.;D. W. Pumplin;Chengsi Yu;R. Christy;David V. Smith
G蛋白亚基α-味蛋白与视杆细胞转导蛋白相似，参与甜味和甜味物质的转导。在啮齿类动物中，不同人群的味蕾对甜味和苦味刺激的敏感性存在差异。大鼠真菌状味蕾对盐的反应比对甜的刺激更敏感，而味觉上的味蕾主要对甜的物质反应。相比之下，仓鼠菌状味蕾对甜味刺激更敏感。这两个物种的卵形和叶状乳头中的味蕾对苦味化合物敏感。这些敏感性的差异应该反映在不同味蕾群体中含味蛋白细胞的数量上。我们检查了大鼠和仓鼠味蕾对α-味觉蛋白抗体的免疫反应性。通过共聚焦显微镜检查标记的味细胞的免疫荧光，并对细胞进行计数。味蛋白阳性细胞被认为是在所有的味蕾区域，他们是纺锤形的，圆形的横截面和顶端的过程，延伸到味孔。在大鼠的腭状味蕾中具有这种特征性形状的细胞是II型（亮）细胞。在大鼠中，菌状乳头的味蕾具有比其他区域更少的gustducin阳性细胞（3.1个/味蕾），包括舌后和腭（>8.9个/味蕾）。蕈状味蕾含有两倍的gustducin表达细胞（6.8/味蕾）的大鼠。这些数据支持α-味蛋白参与哺乳动物味觉受体细胞对甜味和甜味刺激的转导的假设。
The G-protein subunit α-gustducin, which is similar to rod transducin, has been implicated in the transduction of both sweet- and bitter-tasting substances. In rodents, there are differences in sensitivity to sweet and bitter stimuli in different populations of taste buds. Rat fungiform taste buds are more responsive to salts than to sweet stimuli, whereas those on the palate respond predominantly to sweet substances. In contrast, hamster fungiform taste buds are more sensitive to sweet-tasting stimuli. Taste buds in the vallate and foliate papillae of both species are sensitive to bitter compounds. These differences in sensitivity should be reflected in the numbers of gustducin-containing cells in different taste bud populations. We examined taste buds in the rat and hamster for immunoreactivity to an antibody against α-gustducin. Immunofluorescence of labeled taste cells was examined by confocal microscopy, and the cells were counted. Gustducin-positive cells were seen in all taste bud regions; they were spindle-shaped, with circular cross-sections and apical processes that extended to the taste pore. Cells with this characteristic shape in rat vallate taste buds are Type II (light) cells. In the rat, taste buds of the fungiform papillae had fewer gustducin-positive cells (3.1/taste bud) than those of other regions, including the posterior tongue and palate (>8.9/taste bud). Hamster fungiform taste buds contained twice as many gustducin-expressing cells (6.8/taste bud) as those of the rat. These data support the hypothesis that α-gustducin is involved in the transduction of both sweet- and bitter-tasting stimuli by mammalian taste receptor cells.
脊椎动物味觉受体的细胞生物学。
影响因子：
13.9
作者：
Roper,SD
通讯作者：
Roper,SD
在孤束核中编码甜味：大鼠前舌和鼻切管味觉受体的不同作用。
影响因子：
2.5
作者：
Travers,SP;Norgren,R
通讯作者：
Norgren,R