A role for nonsense-mediated mRNA decay in plants: pathogen responses are induced in Arabidopsis thaliana NMD mutants.
A role for nonsense-mediated mRNA decay in plants: pathogen responses are induced in Arabidopsis thaliana NMD mutants.
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DOI：
10.1371/journal.pone.0031917
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Davies B
中科院分区：
文献类型：
--
作者：
Rayson S;Arciga-Reyes L;Wootton L;De Torres Zabala M;Truman W;Graham N;Grant M;Davies B
无义介导的mRNA衰变（NMD）是靶向异常mRNA进行破坏的保守机制。NMD还被发现调节多种生物体中大量基因的表达，尽管其生物学作用尚不清楚，并且已经鉴定出很少的进化上保守的靶标。对三个NMD缺陷的拟南芥系的表达分析表明，绝大多数NMD靶向转录本与对病原体的反应有关。相应地，NMD突变体，其中这些转录物升高，赋予对假单胞菌的部分抗性。这些研究结果表明，NMD在植物中的基因表达调控的生物学原理，并建议操纵NMD可以提供一种新的方法来保护作物。在少数非病原体响应NMD靶向基因中，发现一种潜在的NMD靶向信号，即进化上保守的上游开放阅读框（CuORF），被大量过度表达，这提高了该特征可用于靶向特定生理mRNA以供NMD控制的可能性。
Nonsense-mediated mRNA decay (NMD) is a conserved mechanism that targets aberrant mRNAs for destruction. NMD has also been found to regulate the expression of large numbers of genes in diverse organisms, although the biological role for this is unclear and few evolutionarily conserved targets have been identified. Expression analyses of three Arabidopsis thaliana lines deficient in NMD reveal that the vast majority of NMD-targeted transcripts are associated with response to pathogens. Congruently, NMD mutants, in which these transcripts are elevated, confer partial resistance to Pseudomonas syringae. These findings suggest a biological rationale for the regulation of gene expression by NMD in plants and suggest that manipulation of NMD could offer a new approach for crop protection. Amongst the few non-pathogen responsive NMD-targeted genes, one potential NMD targeted signal, the evolutionarily conserved upstream open reading frame (CuORF), was found to be hugely over-represented, raising the possibility that this feature could be used to target specific physiological mRNAs for control by NMD.
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