Identification, Confirmation, and Replication of Novel Urinary MicroRNA Biomarkers in Lupus Nephritis and Diabetic Nephropathy.
Identification, Confirmation, and Replication of Novel Urinary MicroRNA Biomarkers in Lupus Nephritis and Diabetic Nephropathy.
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狼疮性肾炎和糖尿病肾病中新型尿液 MicroRNA 生物标志物的鉴定、确认和复制。
DOI：
10.1373/clinchem.2017.274175
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发表时间：
2017-09
影响因子：
9.3
通讯作者：
Vaidya VS
中科院分区：
文献类型：
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作者：
Cardenas-Gonzalez M;Srivastava A;Pavkovic M;Bijol V;Rennke HG;Stillman IE;Zhang X;Parikh S;Rovin BH;Afkarian M;de Boer IH;Himmelfarb J;Waikar SS;Vaidya VS
慢性肾脏疾病（CKD）的患病率正在增加，导致显著的发病率和死亡率。肾活检仍然是诊断CKD潜在病因的金标准，但该手术存在并发症风险。本研究的目的是在经活检证实的CKD患者中鉴定与肾功能和组织病理学相关的新的非侵入性生物标志物。我们分析了2402种尿microRNA（miRNAs），以鉴定和确认与糖尿病肾病（n = 58）或狼疮性肾炎（n = 89）患者（CKD的重要病因）的肾功能和组织病理学相关的差异表达的miRNAs，并与健康对照组（分别为n = 93和119）进行比较。然后在2个独立的多机构队列中测量表现最好的miRNA，所述队列为患有糖尿病伴（n = 74）或不伴肾病（n = 71）和系统性红斑狼疮伴（n = 86）或不伴（n = 37）肾炎的患者。在糖尿病肾病患者中，miR-2861、miR-1915- 3 p和miR-4532下调（>10倍，P < 0.0001），并与估计的肾小球滤过率（P < 0.01）和间质纤维化/肾小管萎缩（P < 0.05）相关。miR-2861、miR-1915- 3 p和miR-4532的c统计量分别为0.91、0.86和0.85。在狼疮性肾炎患者中，miR-3201和miR-1273 e下调（>3倍，P < 0.0001），并与毛细血管内肾小球炎症相关（P < 0.01），c统计值分别为0.97和0.91。我们已经鉴定了与组织病理学病变和肾损伤的功能标志物相关的新型miRNA，以促进糖尿病肾病和狼疮性肾炎的敏感性、特异性和非侵入性检测。
The prevalence of chronic kidney disease (CKD) is increasing, leading to significant morbidity and mortality. Kidney biopsy remains the gold standard for diagnosing the underlying etiology of CKD, but the procedure carries complication risks. The aim of this study was to identify novel noninvasive biomarkers correlating with kidney function and histopathology in biopsyproven CKD patients. We profiled 2402 urinary microRNAs (miRNAs) to identify and confirm differentially expressed miRNAs associated with kidney function and histopathology in patients with diabetic nephropathy (n = 58) or lupus nephritis (n = 89), important etiologies of CKD, compared with healthy controls (n = 93 and 119, respectively). Top performing miRNAs were then measured in 2 independent multi-institutional cohorts of patients with diabetes mellitus with (n = 74) or without nephropathy (n = 71) and systemic lupus erythematosus with (n = 86) or without (n = 37) nephritis. In patients with diabetic nephropathy, miR-2861, miR-1915-3p, and miR-4532 were down-regulated (>10-fold, P < 0.0001) and were associated with estimated glomerular filtration rate (P < 0.01) and interstitial fibrosis/tubular atrophy (P < 0.05). The c-statistics for miR-2861, miR-1915-3p, and miR-4532 were 0.91, 0.86, and 0.85, respectively. In lupus nephritis patients, miR-3201 and miR-1273e were down-regulated (>3-fold, P < 0.0001) and associated with endocapillary glomerular inflammation (P < 0.01), with c-statistics of 0.97 and 0.91, respectively. We have identified novel miRNAs that correlate with histopathological lesions and functional markers of kidney damage to facilitate sensitive, specific, and noninvasive detection of diabetic nephropathy and lupus nephritis.
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影响因子：
19.6
作者：
通讯作者：
--
影响因子：
9.3
作者：
Ramachandran K;Saikumar J;Bijol V;Koyner JL;Qian J;Betensky RA;Waikar SS;Vaidya VS
通讯作者：
Vaidya VS
影响因子：
168.9
作者：
Jha, Vivekanand;Garcia-Garcia, Guillermo;Yang, Chih-Wei
通讯作者：
Yang, Chih-Wei
影响因子：
6.1
作者：
Sole, Cristina;Cortes-Hernandez, Josefina;Ordi-Ros, Josep
通讯作者：
Ordi-Ros, Josep
DOI：
10.2215/cjn.03400412
发表时间：
2013-01-01
影响因子：
9.8
作者：
Giannico, Giovanna;Fogo, Agnes B.
通讯作者：
Fogo, Agnes B.