Autocrine and Paracrine Effects of Vascular Endothelial Cells Promote Cutaneous Wound Healing.
Autocrine and Paracrine Effects of Vascular Endothelial Cells Promote Cutaneous Wound Healing.
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血管内皮细胞的自分泌和旁分泌作用促进皮肤伤口愈合
DOI：
10.1155/2021/6695663
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发表时间：
2021
影响因子：
--
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Lu Y;Yang Y;Xiao L;Li S;Liao X;Liu H
当血管内皮细胞受到外界刺激时，旁分泌激素和细胞因子作用于邻近细胞。细胞生物学行为的调节与器官功能的维持和疾病的发生、发展密切相关。然而，尚不清楚血管内皮细胞是否通过自分泌和旁分泌机制影响参与伤口修复的细胞的生物学行为，并最终在伤口愈合中发挥作用。我们的目的是验证血管内皮细胞的自分泌和旁分泌功能对伤口愈合的影响。 采用ELISA法检测人脐血管内皮细胞条件培养基（HUVEC-CM）中血小板源性生长因子、碱性成纤维细胞生长因子、表皮生长因子和血管内皮生长因子。使用不同浓度的HUVEC-CM来处理不同的干细胞。采用CCK-8和划痕实验检测各细胞的增殖和迁移能力。建立小鼠背侧全层皮肤缺损模型，局部注射HUVEC-CM、内皮细胞培养基（ECM）或生理盐水后观察皮肤创面愈合情况。 H&E染色和免疫荧光观察创面组织大体形态、上皮细胞迁移距离、CD3、CD31表达情况。 HUVEC-CM 促进表皮干细胞、皮肤成纤维细胞、骨髓间充质干细胞和 HUVEC 本身的增殖和迁移。此外，HUVEC-CM可以促进小鼠皮肤伤口血管生成和肉芽组织形成，并能加速伤口表面上皮化和胶原蛋白合成，从而促进伤口愈合。 我们的结果清楚地表明，在小鼠模型中利用血管内皮细胞分泌的细胞因子促进伤口愈合是可行且有效的。
When vascular endothelial cells are subjected to external stimuli, paracrine hormones and cytokines act on adjacent cells. The regulation of the biological behaviour of cells is closely related to the maintenance of organ function and the occurrence and development of disease. However, it is unclear whether vascular endothelial cells affect the biological behaviour of cells involved in wound repair through autocrine and paracrine mechanisms and ultimately play a role in wound healing. We aimed to verify the effect of the autocrine and paracrine functions of vascular endothelial cells on wound healing. ELISA was used to detect platelet-derived growth factor, basic fibroblast growth factor, epidermal growth factor, and vascular endothelial growth factor in human umbilical vascular endothelial cell-conditioned medium (HUVEC-CM). Different concentrations of HUVEC-CM were used to treat different stem cells. CCK-8 and scratch assays were used to detect the proliferation and migration ability of each cell. A full-thickness dorsal skin defect model was established in mice, and skin wound healing was observed after the local injection of HUVEC-CM, endothelial cell medium (ECM), or normal saline. H&E staining and immunofluorescence were used to observe the gross morphology of the wound tissue, the epithelial cell migration distance, and the expression of CD3 and CD31. HUVEC-CM promotes the proliferation and migration of epidermal stem cells, skin fibroblasts, bone marrow mesenchymal stem cells, and HUVECs themselves. Furthermore, HUVEC-CM can promote angiogenesis in mouse skin wounds and granulation tissue formation and can accelerate wound surface epithelialization and collagen synthesis, thereby promoting wound healing. Our results clearly suggest that it is practicable and effective to promote wound healing with cytokines secreted by vascular endothelial cells in a mouse model.
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影响因子：
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作者：
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作者：
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DOI：
10.1007/s11626-013-9687-0
发表时间：
2014-02-01
影响因子：
2.1
作者：
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通讯作者：
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