Peptide-Modified Chitosan Hydrogels Accelerate Skin Wound Healing by Promoting Fibroblast Proliferation, Migration, and Secretion.
Peptide-Modified Chitosan Hydrogels Accelerate Skin Wound Healing by Promoting Fibroblast Proliferation, Migration, and Secretion.
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肽修饰壳聚糖水凝胶通过促进成纤维细胞增殖、迁移和分泌来加速皮肤伤口愈合
DOI：
10.1177/0963689717721216
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发表时间：
2017-08
影响因子：
3.3
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Chen X;Zhang M;Chen S;Wang X;Tian Z;Chen Y;Xu P;Zhang L;Zhang L;Zhang L
皮肤伤口愈合是一个复杂的过程，涉及多种细胞和细胞因子。成纤维细胞在这一过程中发挥重要作用，参与向肌成纤维细胞的转化、细胞外基质（ECM）和纤维的合成以及多种生长因子的分泌。本研究评估了肽Ser-Ile-Lys-Val-Ala-Val （SIKVAV）修饰的壳聚糖水凝胶对皮肤伤口愈合的影响。我们利用体外成纤维细胞研究了SIKVAV肽促进细胞增殖和迁移、胶原合成和多种生长因子分泌的能力。我们还使用肽sikvav修饰的壳聚糖水凝胶治疗小鼠皮肤伤口。苏木精和伊红染色表明，肽修饰的壳聚糖水凝胶与阴性和阳性对照相比，能促进创面的再上皮化。马松三色染色表明，与阴性和阳性对照相比，肽修饰壳聚糖水凝胶处理的伤口沉积了更多的胶原纤维。免疫组化结果显示肽修饰壳聚糖水凝胶促进皮肤创面血管生成。综上所述，这些结果表明肽sikvav修饰的壳聚糖水凝胶可用于伤口敷料和皮肤伤口的治疗。
Skin wound healing is a complicated process that involves a variety of cells and cytokines. Fibroblasts play an important role in this process and participate in transformation into myofibroblasts, the synthesis of extracellular matrix (ECM) and fibers, and the secretion of a variety of growth factors. This study assessed the effects of peptide Ser-Ile-Lys-Val-Ala-Val (SIKVAV)--modified chitosan hydrogels on skin wound healing. We investigated the capability of peptide SIKVAV to promote cell proliferation and migration, the synthesis of collagen, and the secretion of a variety of growth factors using fibroblasts in vitro. We also treated skin wounds established in mice using peptide SIKVAV-modified chitosan hydrogels. Hematoxylin and eosin staining showed that peptide-modified chitosan hydrogels enhanced the reepithelialization of wounds compared with negative and positive controls. Masson’s trichrome staining demonstrated that more collagen fibers were deposited in the wounds treated with peptide-modified chitosan hydrogels compared with the negative and positive controls. Immunohistochemistry revealed that the peptide-modified chitosan hydrogels promoted angiogenesis in the skin wound. Taken together, these results suggest that peptide SIKVAV-modified chitosan hydrogels may be useful in wound dressing and the treatment of skin wounds.
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DOI：
10.2147/ccid.s50046
发表时间：
2014
期刊：
Clinical, cosmetic and investigational dermatology
影响因子：
--
作者：
Darby IA;Laverdet B;Bonté F;Desmoulière A
通讯作者：
Desmoulière A
层粘连蛋白衍生肽与 Lys 加帽 PLLA 缀合对新生小鼠小脑 C17.2 干细胞的影响
影响因子：
14
作者：
He, Liumin;Liao, Susan;Lu, Jiang
通讯作者：
Lu, Jiang
DOI：
10.1163/092050609x12457417534295
发表时间：
2009-01-01
影响因子：
3.6
作者：
Lefler, Amy;Ghanem, Amyl
通讯作者：
Ghanem, Amyl
影响因子：
4.5
作者：
Han, Yanfu;Tao, Ran;Liu, Jing
通讯作者：
Liu, Jing
影响因子：
8
作者：
Bielefeld, Kirsten A.;Amini-Nik, Saeid;Alman, Benjamin A.
通讯作者：
Alman, Benjamin A.