Detection of Adult T‐Cell Leukemia Virus (ATLV) Bearing Lymphocytes in Concentrated Red Blood Cells Derived from ATL Associated Antibody (ATLA‐Ab) Positive Donors
Detection of Adult T‐Cell Leukemia Virus (ATLV) Bearing Lymphocytes in Concentrated Red Blood Cells Derived from ATL Associated Antibody (ATLA‐Ab) Positive Donors
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检测来自 ATL 相关抗体 (ATLA-Ab) 阳性供体的浓缩红细胞中携带成人 T 细胞白血病病毒 (ATLV) 的淋巴细胞
DOI：
10.1111/j.1423-0410.1986.tb04883.x
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发表时间：
1986
期刊：
影响因子：
2.7
通讯作者：
Tsuneo Fukuda
中科院分区：
文献类型：
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作者：
Y. Morishima;Ken‐ichi Ohya;R. Ueda;Tsuneo Fukuda
摘要。采用间接免疫荧光法（IF）筛选成人T细胞白血病相关抗体（ATLA‐Ab）阳性人群。从浓缩红细胞（CRC）中收集淋巴细胞，在体外加和不加植物血凝素（PHA）培养10天。采用ATL病毒p19核心蛋白反应小鼠单克隆抗体ATL - 19， IF法分析ATL病毒（ATLV）阳性淋巴细胞在体外培养过程中的表达情况。经PHA培养10天后，97%的ATLA‐Ab阳性CRC（36例）显示出ATLV阳性淋巴细胞，而ATLA‐Ab阴性CRC（22例）没有一例显示出ATLV阳性淋巴细胞。保存2、4和7天的10例ATLA‐Ab阳性CRC均含有体外培养后表达ATLV的淋巴细胞，而保存14天的10例CRC中有7例表达ATLV阳性淋巴细胞，保存20天的10例CRC中只有1例表达ATLV阳性淋巴细胞。这些数据表明，几乎所有的ATLA‐Ab阳性血液中都含有ATLV阳性淋巴细胞，并且这些ATLV阳性淋巴细胞的体外外观在CRC储存超过14天后会减少。
Abstract. Adult T cell leukemia associated antibody (ATLA‐Ab) positive persons were screened by indirect immunofluorescence (IF) testing. Their lymphocytes were collected from concentrated red blood cells (CRC), and cultured in vitro with and without phytohemagglutinin (PHA) for 10 days. The expression of ATL virus (ATLV) positive lymphocytes during the in vitro culture was then analyzed by IF assay using mouse monoclonal antibody ATL‐19 reactive to p19 core protein of ATLV. 97% of ATLA‐Ab positive CRC (36 cases) demonstrated ATLV positive lymphocytes after being cultured for more than 10 days with PHA, whereas, none of ATLA‐Ab negative CRC (22 cases) demonstrated ATLV positive lymphocytes. All of the 10 ATLA‐Ab positive CRC that were stored for 2, 4, and 7 days contained lymphocytes which expressed ATLV after in vitro culture, while 7 of 10 CRC stored for 14 days and only 1 of 10 CRCs stored for 20 days, expressed ATLV positive lymphocytes. This data indicates that almost all of the ATLA‐Ab positive blood contained ATLV positive lymphocytes, and that the in vitro appearance of these ATLV positive lymphocytes was reduced by storing the CRC for more than 14 days.
DOI：
10.1073/pnas.79.6.2031
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
YOSHIDA, M;MIYOSHI, I;HINUMA, Y
通讯作者：
HINUMA, Y