Oxyhemoglobin-induced cytotoxicity and arachidonic acid release in cultured bovine endothelial cells.
Oxyhemoglobin-induced cytotoxicity and arachidonic acid release in cultured bovine endothelial cells.
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培养的牛内皮细胞中氧合血红蛋白诱导的细胞毒性和花生四烯酸释放。
作者：
Takenaka,K;Kassell,NF;Foley,PL;Lee,KS
内皮功能受损与蛛网膜下腔出血后的血管痉挛有关。氧合血红蛋白被认为是血管痉挛发病机制的关键触发因素。本研究检测了氧合血红蛋白对培养的牛颈动脉内皮细胞的直接影响。用氧合血红蛋白处理汇合的内皮细胞，并研究了以下内容：1）细胞形态，2）细胞密度，3）[3H]花生四烯酸处理的细胞中放射性标记的释放。暴露于氧合血红蛋白的内皮细胞表现出脱离空泡，细胞密度显着降低。时间和剂量依赖性方式。超氧化物歧化酶是一种自由基清除剂，可部分防止氧合血红蛋白的细胞毒性作用。氧合血红蛋白以时间和剂量依赖性方式增加[3H]花生四烯酸处理的细胞中放射性标记的释放。用磷脂酶 A2 抑制剂或钙螯合剂处理可抑制氧合血红蛋白对花生四烯酸释放和细胞活力的影响。氧合血红蛋白对培养的内皮细胞产生直接细胞毒性作用，这种作用与 [3H]花生四烯酸标记细胞的释放增加有关。磷脂酶 A2 和自由基似乎参与内皮细胞损伤的发病机制。氧合血红蛋白诱导的内皮细胞受损可能导致脑血管病理。
An impairment of endothelial function is associated with vasospasm after subarachnoid hemorrhage. Oxyhemoglobin is considered to be a critical trigger in the pathogenesis of vasospasm. The present studies examined the direct effects of oxyhemoglobin on cultured endothelial cells from bovine carotid artery.Confluent endothelial cells were treated with oxyhemoglobin, and the following were studied: 1) cell morphology, 2) cell density, and 3) the release of radiolabel from [3H]arachidonic acid-treated cells.Endothelial cells exposed to oxyhemoglobin exhibited detachment vacuoles, and cell density was significantly decreased in time- and dose-dependent manners. Superoxide dismutase, a free radical scavenger, provided partial protection against the cytotoxic effects of oxyhemoglobin. The release of radiolabel from [3H]arachidonic acid-treated cells was increased by oxyhemoglobin in time- and dose-dependent manners. Treatment with an inhibitor of phospholipase A2 or a calcium chelator inhibited the effects of oxyhemoglobin on arachidonic acid release and cellular viability.Oxyhemoglobin exerts a direct cytotoxic effect on cultured endothelial cells, and this effect is associated with increased release from [3H]arachidonic acid-labeled cells. Phospholipase A2 and free radicals appear to participate in the pathogenesis of endothelial cell damage. Oxyhemoglobin-induced compromise of endothelial cells may contribute to cerebrovascular pathology.
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蛛网膜下腔出血后各种大小示踪剂对血动脉壁屏障的破坏
影响因子：
2.4
作者：
T. Nakagomi;N. Kassell;H. Johshita;R. Lehman;S. Fujiwara;Tsutomu Sasaki
通讯作者：
Tsutomu Sasaki
DOI：
10.1016/0005-2744(73)90180-0
发表时间：
1973-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
WEVER, R;VANGELDE.BF
通讯作者：
VANGELDE.BF
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影响因子：
2.9
作者：
G. Massicotte;M. Oliver;G. Lynch;M. Baudry
通讯作者：
M. Baudry
DOI：
--
发表时间：
1985-03
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
通讯作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
体外平滑肌实验与脑血管痉挛的相关性。
影响因子：
8.3
作者：
Wellum,GR;Peterson,JW;Zervas,NT
通讯作者：
Zervas,NT