Methods for recording and measuring tonic GABAA receptor-mediated inhibition.
Methods for recording and measuring tonic GABAA receptor-mediated inhibition.
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DOI：
10.3389/fncir.2013.00193
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发表时间：
2013-12-05
影响因子：
3.5
通讯作者：
Smart TG
中科院分区：
文献类型：
--
作者：
Bright DP;Smart TG
由GABAA受体介导的紧张性抑制电导现已在许多不同的脑区被识别和表征。大多数强直性GABA能抑制的实验研究都是使用急性脑片标本进行的，但在各种不同的条件下都记录到了强直性电流。这种记录条件的多样性可能会影响许多负责控制紧张抑制的因素，并可能使不同研究之间的比较变得困难。在这篇综述中，我们将首先考虑各种实验条件，包括动物年龄、记录温度和溶液组成，可能会影响紧张性GABA的电导。然后，我们将考虑与如何测量和随后分析张力电导有关的一些技术考虑，包括使用电流噪声如何为量化张力电流的变化提供一种补充和可靠的方法。
Tonic inhibitory conductances mediated by GABAA receptors have now been identified and characterized in many different brain regions. Most experimental studies of tonic GABAergic inhibition have been carried out using acute brain slice preparations but tonic currents have been recorded under a variety of different conditions. This diversity of recording conditions is likely to impact upon many of the factors responsible for controlling tonic inhibition and can make comparison between different studies difficult. In this review, we will firstly consider how various experimental conditions, including age of animal, recording temperature and solution composition, are likely to influence tonic GABAA conductances. We will then consider some technical considerations related to how the tonic conductance is measured and subsequently analyzed, including how the use of current noise may provide a complementary and reliable method for quantifying changes in tonic current.
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DOI：
10.1523/jneurosci.0460-12.2012
发表时间：
2012-08-08
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Duguid I;Branco T;London M;Chadderton P;Häusser M
通讯作者：
Häusser M
影响因子：
5.5
作者：
Amato, A;Connolly, CN;Smart, TG
通讯作者：
Smart, TG
影响因子：
4.7
作者：
Atack, John R.;Bayley, Peter J.;Dawson, Gerard R.
通讯作者：
Dawson, Gerard R.
影响因子：
4.7
作者：
Casula, MA;Bromidge, FA;Hadingham, KL
通讯作者：
Hadingham, KL
影响因子：
3.3
作者：
de Groote, L.;Linthorst, A. C. E.
通讯作者：
Linthorst, A. C. E.