Recent Progress in the Regeneration and Genetic Transformation System of Cucumber

Recent Progress in the Regeneration and Genetic Transformation System of Cucumber
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黄瓜再生及遗传转化体系研究进展

DOI:
10.3390/app12147180
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发表时间:
2022-07
期刊:
影响因子:
--
通讯作者:
Peng Zhang
Peng Zhang
中科院分区:
--
文献类型:
--
作者:
Jihong Tan;Lili Lin;Haiyan Luo;Shengjun Zhou;Yuqiang Zhu;Xin Wang;Li Miao;Huasen Wang;Peng Zhang

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黄瓜(Cucumis sativus L.)属于葫芦科(葫芦科),是中国主要蔬菜作物之一。常规的遗传育种方法在短期内无法提高黄瓜对各种环境胁迫和病虫害的耐受性,而生物工程技术可以应用于黄瓜育种,培育出高产优质的新品种。再生和遗传转化系统是现代黄瓜育种的关键技术。与再生体系相比,遗传转化体系尚未完全发挥作用,遗传转化效率低是黄瓜栽培的瓶颈。本文系统综述了影响黄瓜植株再生和遗传转化的关键因素,包括基因型选择、外植体来源、外源激素添加方式、转基因引种和共培养方法、选择方法等。此外,我们还重点介绍了利用遗传转化技术研究重要农艺性状的分子机制的最新进展,如果实长度、果实疣和花发育。为今后黄瓜品种改良研究提供参考信息。
Cucumber (Cucumis sativus L.), belonging to the gourd family (Cucurbitaceae), is one of the major vegetable crops in China. Conventional genetic breeding methods are ineffective for improving the tolerance of cucumber to various environmental stresses, diseases, and pests in the short term, but bio-engineering technologies can be applied to cucumber breeding to produce new cultivars with high yield and quality. Regeneration and genetic transformation systems are key technologies in modern cucumber breeding. Compared with regeneration systems, genetic transformation systems are not yet fully effective, and the low efficiency of genetic transformation is a bottleneck in cucumber cultivation. Here, we systematically review the key factors influencing the regeneration and genetic transformation of cucumber plants, including the selection of genotype, source of explants and forms of exogenous hormones added to the medium, the methods of transgene introduction and co-cultivation, and selection methods. In addition, we also focus on recent advances in the study of molecular mechanisms underlying important agronomic traits using genetic transformation technology, such as fruit length, fruit warts, and floral development. This review provides reference information for future research on improvements in cucumber varieties.
DOI: 10.16420/j.issn.0513-353x.2020-0376
发表时间: 2020-09
期刊: --
影响因子: --
作者:
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