[Roles of Pseudomonas aeruginosa-derived proteases as a virulence factor].
[Roles of Pseudomonas aeruginosa-derived proteases as a virulence factor].
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[铜绿假单胞菌来源的蛋白酶作为毒力因子的作用]。
DOI：
10.3412/jsb.68.313
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Yutaka Kida
中科院分区：
文献类型：
--
作者：
Yutaka Kida
铜绿假单胞菌产生的主要毒力因子包括破坏宿主组织的分泌性蛋白酶。在分析的蛋白酶中，碱性蛋白酶（AprA）和弹性蛋白酶B（LasB）已被广泛表征。尽管铜绿假单胞菌蛋白质数据库预测了其他几种潜在的蛋白酶的存在，但对参与该生物体致病性的蛋白酶知之甚少。在这项研究中，我们发现铜绿假单胞菌产生一种新的大细胞外蛋白酶（LepA），不同于已知的蛋白酶，如AprA和LasB。LepA的序列分析显示了通过双伴侣分泌途径转运的蛋白质的分子未来。我们证明LepA可以通过蛋白酶激活受体-1、-2或-4激活NF-κ B驱动的启动子。另一方面，蛋白酶的功能之一是通过降解宿主酶或甚至通过引起组织损伤以促进细菌的存活来水解蛋白质和肽以获得营养。因此，为了研究LepA在铜绿假单胞菌体内毒力和生长中的作用，我们使用铜绿假单胞菌急性全身感染的小鼠模型比较了野生型菌株及其突变体的毒力和生长。我们的研究结果表明，LepA有助于铜绿假单胞菌的体内毒力和生长。
The major virulence factors produced by Pseudomonas aeruginosa include secreted proteases that damage host tissues. Of the proteases analyzed, alkaline protease (AprA) and elastase B (LasB) have been characterized extensively. Although P. aeruginosa protein database predicts the presence of several other potential proteases, little has been known about the proteases involving in the pathogenicity of this organism. In this study, we found that P. aeruginosa produces a novel large extracellular protease (LepA) distinct from known proteases such as AprA and LasB. Sequence analysis of LepA showed a molecular future of the proteins transported by the two-partner secretion pathway. We demonstrated that LepA can activate NF-kB-driven promoter through protease-activated receptor-1, -2 or -4. On the other hand, one of the functions of proteases is to hydrolyze proteins and peptides for nutrient acquisition either by degrading host enzymes or even by causing tissue damage to further the survival of the bacterium. Therefore, to investigate the role of LepA in in vivo virulence and growth of P. aeruginosa, we compared the virulence and growth of a wild-type strain and its mutant using a mouse model of acute systemic infection by P. aeruginosa. Our results suggest that LepA contributes to the in vivo virulence and growth of P. aeruginosa.
影响因子：
64.8
作者：
Stover, CK;Pham, XQ;Olson, MV
通讯作者：
Olson, MV
影响因子：
3.4
作者：
Kida, Yutaka;Higashimoto, Yuichiro;Kuwano, Koichi
通讯作者：
Kuwano, Koichi