Quorum Sensing Communication Modules for Microbial Consortia.
Quorum Sensing Communication Modules for Microbial Consortia.
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DOI：
10.1021/acssynbio.5b00286
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发表时间：
2016-09-16
影响因子：
4.7
通讯作者：
Hasty J
中科院分区：
文献类型：
--
作者：
Scott SR;Hasty J
单一工程生物体的力量受到其基因改造能力的限制。为了规避任何单一微生物的限制，合成生物学的一个新前沿正在出现：合成生态学，或微生物财团的工程。在这里，我们为这样的联盟开发通信系统，以允许社区中不同成员之间的复杂社会行为。我们认为，如果社区成员之间的交流得到很好的管理，这些社区在执行复杂任务的能力方面将超过单一文化。群体感应因其巨大的多样性和在合成生物学中的既定用途而被认为是精确控制工程微生物生态系统最有希望的候选方法。通过启动子和蛋白质修饰，我们设计了两个群体感应系统（rpa和tra），以添加到广泛使用的lux和las系统中。通过测试所有系统之间的串扰，我们彻底表征了许多新的诱导系统，用于工程化社区的通用控制。此外，我们已经确定了几个系统对，表现出有用的正交性类型。最值得注意的是，tra和rpa系统彼此之间既没有信号串扰，也没有启动子串扰，使它们在操作中完全正交。总的来说，通过表征所有四个系统及其组件之间的相互作用，这些电路应该适合于在微生物财团中使用的更高层次的遗传电路。
The power of a single engineered organism is limited by its capacity for genetic modification. To circumvent the constraints of any singular microbe, a new frontier in synthetic biology is emerging: synthetic ecology, or the engineering of microbial consortia. Here we develop communication systems for such consortia in an effort to allow for complex social behavior across different members of a community. We posit that such communities will outpace monocultures in their ability to perform complicated tasks if communication among and between members of the community is well regulated. Quorum sensing was identified as the most promising candidate for precise control of engineered microbial ecosystems, due to its large diversity and established utility in synthetic biology. Through promoter and protein modification, we engineered two quorum sensing systems (rpa and tra) to add to the extensively used lux and las systems. By testing the cross-talk between all systems, we thoroughly characterized many new inducible systems for versatile control of engineered communities. Furthermore, we’ve identified several system pairs that exhibit useful types of orthogonality. Most notably, the tra and rpa systems were shown to have neither signal crosstalk nor promoter crosstalk for each other, making them completely orthogonal in operation. Overall, by characterizing the interactions between all four systems and their components, these circuits should lend themselves to higher-level genetic circuitry for use in microbial consortia.
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