Expression of Different Isoforms of Versican During the Development of Mouse Mandibular First Molars
Expression of Different Isoforms of Versican During the Development of Mouse Mandibular First Molars
复制标题
小鼠下颌第一磨牙发育过程中Versacan不同异构体的表达
DOI：
10.1369/0022155419846875
复制
发表时间：
2019-04
影响因子：
3.2
通讯作者：
Jiang Beizhan
中科院分区：
文献类型：
--
作者：
Xu Fangfang;Cai Wenping;Chen Weiting;Li Lefeng;Li Xuyan;Jiang Beizhan
Versican是一种富含细胞外基质的大型硫酸软骨素蛋白聚糖，它至少有四种不同的亚型，称为V0，V1，V2和V3。尽管多功能蛋白聚糖在多种发育器官中稳定表达，但多功能蛋白聚糖异构体在牙齿发育过程中的表达尚不清楚。因此，本研究旨在探讨多功能蛋白聚糖亚型在小鼠磨牙发育过程中的表达。采用免疫组织化学方法检测胎龄11.5天至出生后21天的下颌第一磨牙多功能蛋白聚糖（versican，Vcan）亚型的表达，并采用实时荧光定量PCR方法检测胎龄13.5天至出生后7天的下颌第一磨牙多功能蛋白聚糖（versican，Vcan）亚型的基因表达。结果表明，多功能蛋白聚糖亚型的表达模式不同，星状网状细胞（SR）和邻近上皮根鞘的间充质细胞（HERS）仅表达V1，成熟成牙本质细胞主要表达V2，而牙乳头和成釉细胞可能同时表达V0/V1/V2。这些结果表明，不同的versican亚型可能在牙齿发育中发挥不同的作用，我们推测，V0/V1可能与细胞增殖密切相关，而V2与细胞分化有关。
Versican is a large chondroitin sulfate proteoglycan enriched in the extracellular matrix, and it has at least four different isoforms, termed V0, V1, V2, and V3. Although several studies have demonstrated that versican is stably expressed in various developing organs, the expression of versican isoforms during tooth development has not been elucidated yet. Therefore, the present study was to investigate the expression of versican isoforms in the developing mouse molars. The mandibular first molars from embryonic day (E) 11.5 to postnatal day (PN) 21 were used to investigate the expression of versican isoforms by immunohistochemistry, and the gene expressions of versican (Vcan) isoforms from E13.5 to PN7 were analyzed by quantitative real-time PCR. The results exhibited different expressing patterns of versican isoforms—the stellate reticulum (SR) and the dental mesenchymal cells adjacent to Hertwig’s Epithelial Root Sheath (HERS) only expressed V1 and the mature odontoblasts mainly expressed V2, while the dental papilla and the ameloblasts might both express V0/V1/V2. These results suggested that different versican isoforms may act different roles in the tooth development, and we speculated that V0/V1 might be intimately involved in the cell proliferation while V2 was associated in the cytodifferentiation.
登录
查看更多内容
DOI：
10.4081/ejh.2009.e15
发表时间：
2009-09
期刊：
European Journal of Histochemistry : EJH
影响因子：
--
作者：
Alessandra Ruggeri;Giovanna Orsini;A. Mazzoni;F. Nato;Veronica Papa;M. Piccirilli;A. Putignano;Giovanni Mazzotti;E. D. S. Dorigo;Lorenzo Breschi
通讯作者：
Alessandra Ruggeri;Giovanna Orsini;A. Mazzoni;F. Nato;Veronica Papa;M. Piccirilli;A. Putignano;Giovanni Mazzotti;E. D. S. Dorigo;Lorenzo Breschi
影响因子：
5
作者：
P. Bartold;A. Moule;H. Li;P. Rigby
通讯作者：
P. Bartold;A. Moule;H. Li;P. Rigby
影响因子：
5
作者：
Miquel-Serra, Laia;Serra, Montserrat;Bassols, Anna
通讯作者：
Bassols, Anna
影响因子：
4.8
作者：
Dutt, S;Kléber, M;Zimmermann, DR
通讯作者：
Zimmermann, DR
影响因子：
7.6
作者：
S. Yamauchi;Hui Cheng;Peter J. Neame;Bruce Caterson;Mitsuo Yamauchi
通讯作者：
S. Yamauchi;Hui Cheng;Peter J. Neame;Bruce Caterson;Mitsuo Yamauchi