Resonator nanophotonic standing-wave array trap for single-molecule manipulation and measurement.
Resonator nanophotonic standing-wave array trap for single-molecule manipulation and measurement.
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DOI：
10.1038/s41467-021-27709-3
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发表时间：
2022-01-10
影响因子：
16.6
通讯作者：
Wang MD
中科院分区：
文献类型：
--
作者：
Ye F;Inman JT;Hong Y;Hall PM;Wang MD
纳米光子镊子代表了在芯片上并行操作和测量单个生物分子方面具有重大潜力的新兴平台。然而，捕集力的产生是其广泛应用的一个重大障碍。在这里，我们提出了一个谐振器纳米光子驻波阵列陷阱（谐振器- nswat），显示出显著的力增强。该平台将临界耦合谐振器设计集成到nSWAT中，并集成了一种新颖的陷阱复位方案。nSWAT现在可以进行标准的单分子实验，包括拉伸DNA分子来测量它们的力延伸关系，解压缩DNA分子，破坏和绘制蛋白质-DNA相互作用。这些实验已经实现了20 pN量级的捕获力，同时通过对多个分子并行进行的测量证明了碱基对的分辨率。因此，谐振器- nswat平台现在在力产生和分辨率方面满足台式精密光学捕获仪器的基准。这代表了用于此类单分子实验的纳米光子平台的首次演示。纳米光子镊的应用受到低捕获力的限制。在这里，作者通过集成临界耦合谐振器设计，展示了纳米光子驻波阵列陷阱中增强的力产生，并演示了常见的单分子实验。
Nanophotonic tweezers represent emerging platforms with significant potential for parallel manipulation and measurements of single biological molecules on-chip. However, trapping force generation represents a substantial obstacle for their broader utility. Here, we present a resonator nanophotonic standing-wave array trap (resonator-nSWAT) that demonstrates significant force enhancement. This platform integrates a critically-coupled resonator design to the nSWAT and incorporates a novel trap reset scheme. The nSWAT can now perform standard single-molecule experiments, including stretching DNA molecules to measure their force-extension relations, unzipping DNA molecules, and disrupting and mapping protein-DNA interactions. These experiments have realized trapping forces on the order of 20 pN while demonstrating base-pair resolution with measurements performed on multiple molecules in parallel. Thus, the resonator-nSWAT platform now meets the benchmarks of a table-top precision optical trapping instrument in terms of force generation and resolution. This represents the first demonstration of a nanophotonic platform for such single-molecule experiments. Applications of nanophotonic tweezers have been limited by the low trapping force. Here, the authors present enhanced force generation in a nanophotonic standing-wave array trap by integrating a critically-coupled resonator design and demonstrate common single-molecule experiments.
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影响因子：
14.8
作者：
通讯作者：
--
影响因子：
10.8
作者：
Inman JT;Smith BY;Hall MA;Forties RA;Jin J;Sethna JP;Wang MD
通讯作者：
Wang MD
影响因子：
64.8
作者：
Anders, Carolin;Niewoehner, Ole;Duerst, Alessia;Jinek, Martin
通讯作者：
Jinek, Martin
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
3.9
作者：
Granéli, A;Yeykal, CC;Greene, EC
通讯作者：
Greene, EC