Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats.
Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats.
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DOI：
10.1016/j.physbeh.2014.03.028
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发表时间：
2014-05-10
影响因子：
2.9
通讯作者：
Shi H
中科院分区：
文献类型：
--
作者：
Liu X;Zhu Z;Kalyani M;Janik JM;Shi H
性别差异存在于能量平衡的调节中，以应对卡路里的缺乏或过剩。脑源性神经营养因子（BDNF）是一种调节能量稳态的厌氧肽。Bdnf mRNA在下丘脑腹内侧核（VMH）的表达与能量和生殖状态密切相关。我们假设Bdnf在VMH中的表达受到雄性和雌性大鼠能量平衡改变的差异调节。通过饮食干预，包括禁食诱导的负能量状态和高脂饮食（HFD）喂养诱导的正能量状态，以及低脂饮食（LFD）喂养和高脂饮食配对喂养（HFD- pf），比较饮食和能量状态的变化对雄性和雌性大鼠VMH Bdnf表达的影响。禁食24小时后，禁食的雄鼠VMH Bdnf表达比喂食的雄鼠低，而雌性没有，这表明禁食的雄鼠减少了Bdnf表达，导致了贪食和体重增加。与LFD雄性大鼠相比，雄性HFD肥胖大鼠和HFD- pf非肥胖大鼠的Bdnf表达也同样减少，这表明Bdnf表达的抑制与喂食高脂肪饮食有关，而不是增加肥胖。高脂肪喂养过程中BDNF信号的减少会增加进食的动力，并可能导致男性饮食引起的肥胖。相比之下，当能量稳态受到干扰时，雌性VMH Bdnf的表达稳定维持。这些结果表明，饮食和能量状态对中枢Bdnf表达的调节存在性别差异。
Sex differences exist in the regulation of energy homeostasis in response to calorie scarcity or excess. Brain-derived neurotrophic factor (BDNF) is one of the anorexigenic neuropeptides regulating energy homeostasis. Expression of Bdnf mRNA in the ventromedial nucleus of the hypothalamus (VMH) is closely associated with energy and reproductive status. We hypothesized that Bdnf expression in the VMH was differentially regulated by altered energy balance in male and female rats. Using dietary intervention, including fasting-induced negative energy status and high-fat diet (HFD) feeding-induced positive energy status, along with low-fat diet (LFD) feeding and HFD pair-feeding (HFD-PF), effects of diets and changes in energy status on VMH Bdnf expression were compared between male and female rats. Fasted males but not females had lower VMH Bdnf expression than their fed counterparts following 24-hour fasting, suggesting that fasted males reduced Bdnf expression to drive hyperphagia and body weight gain. Male HFD obese and HFD-PF non-obese rats had similarly reduced expression of Bdnf compared with LFD males, indicating that dampened Bdnf expression was associated with feeding a diet high in fat instead of increased adiposity. Decreased BDNF signaling during HFD feeding would increase a drive to eat and may contribute to diet-induced obesity in males. In contrast, VMH Bdnf expression was stably maintained in females when energy homeostasis was disturbed. These results suggest sex-distinct regulation of central Bdnf expression by diet and energy status.
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