Optimized DNA Vaccine Enhanced by Adjuvant IL28B Induces Protective Immune Responses Against Herpes Simplex Virus Type 2 in Mice.
Optimized DNA Vaccine Enhanced by Adjuvant IL28B Induces Protective Immune Responses Against Herpes Simplex Virus Type 2 in Mice.
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IL28B 佐剂增强的优化 DNA 疫苗可在小鼠体内诱导针对 2 型单纯疱疹病毒的保护性免疫反应。
DOI：
10.1089/vim.2017.0033
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发表时间：
2017
期刊：
影响因子：
2.2
通讯作者：
W. Kong
中科院分区：
文献类型：
--
作者：
Yan Zhou;Ziyan Wang;Yongqing Xu;Zeqiang Zhang;Rui Hua;Wei Liu;Chunlai Jiang;Yan Chen;Wenying Yang;W. Kong
抗原特异性免疫应答决定了单纯疱疹病毒2型（HSV-2）疫苗的效力。为优化gD 2抗原的免疫原性，本研究构建了编码HSV-2糖蛋白D的gD 2 Δ UL 25 DNA疫苗和编码病毒衣壳顶蛋白的UL 25基因。将gD 2和gD 2 Δ UL 25 DNA疫苗与福尔马林灭活的HSV-2（FI-HSV-2）进行比较，结果显示gD 2 Δ UL 25诱导的保护性免疫应答比gD 2更强。因此，选择gD 2 Δ UL 25以使用IL 28 B佐剂进一步评估。用gD 2 Δ UL 25/IL 28 B免疫引起比单独用gD 2 Δ UL 25更强的体液和T细胞免疫应答。与对照组相比，gD 2 Δ UL 25/IL 28 B降低了HSV-2病毒载量，并诱导了针对HSV-2产生的生殖道病变的保护作用。这些结果表明，与FI-HSV-2相比，IL-28 B佐剂的预防性DNA疫苗gD 2 Δ UL 25可增强体液和T细胞免疫应答，并提高雌性小鼠对HSV-2的保护性免疫应答。
Antigen-specific immune responses determine the efficacy of herpes simplex virus type 2 (HSV-2) vaccines. To optimize the immunogenicity of the antigen gD2, we developed the gD2ΔUL25 DNA vaccine encoding HSV-2 glycoprotein D and UL25 gene encoding viral capsid vertex proteins in this study. The gD2 and gD2ΔUL25 DNA vaccines were compared with formalin-inactivated HSV-2 (FI-HSV-2), and results showed a greater protective immune response induced by gD2ΔUL25 than by gD2. Therefore, gD2ΔUL25 was chosen to evaluate further using the IL28B adjuvant. Immunization with gD2ΔUL25/IL28B elicited stronger humoral and T cell immune responses than with gD2ΔUL25 alone. Compared with controls, gD2ΔUL25/IL28B decreased HSV-2 viral loads and induced protective effects against genital tract lesions generated by HSV-2. These findings demonstrated that the prophylactic DNA vaccine gD2ΔUL25 with IL28B adjuvant could enhance the humoral and T cell immune responses, and improve the protective immune response against HSV-2 in female mice compared with FI-HSV-2.
DOI：
10.1056/nejmoa1103151
发表时间：
2012-01-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
Belshe RB;Leone PA;Bernstein DI;Wald A;Levin MJ;Stapleton JT;Gorfinkel I;Morrow RL;Ewell MG;Stokes-Riner A;Dubin G;Heineman TC;Schulte JM;Deal CD;Herpevac Trial for Women
通讯作者：
Herpevac Trial for Women
DOI：
10.1073/pnas.84.21.7413
发表时间：
1987-11-01
影响因子：
11.1
作者：
FELGNER, PL;GADEK, TR;DANIELSEN, M
通讯作者：
DANIELSEN, M