Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006-2016).
Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006-2016).
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DOI：
10.1038/s41426-018-0190-2
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发表时间：
2018-11-28
影响因子：
13.2
通讯作者：
Perez DR
中科院分区：
文献类型：
--
作者：
Rimondi A;Gonzalez-Reiche AS;Olivera VS;Decarre J;Castresana GJ;Romano M;Nelson MI;van Bakel H;Pereda AJ;Ferreri L;Geiger G;Perez DR
野生水禽是甲型流感病毒的主要宿主。从2006年至2016年在阿根廷的62种鸟类中收集了泄殖腔拭子和粪便样本（n = 6595），并对甲型流感病毒进行了筛查。对来自6种水禽的15种流感分离株进行全基因组测序，发现了先前在南美洲描述的亚型组合（H1N1、H4 N2、H4 N6（n = 3）、H5 N3、H6 N2（n = 4）和H10 N7（n = 2）），以及先前在该地区未发现的新亚型组合（H4 N8、H7 N7和H7N9）。值得注意的是，所有15个阿根廷分离株的内部基因片段都属于南美洲谱系，表明这些病毒在南半球的进化不同。时间尺度的同源性分析表明，南美洲的基因片段在约30年至约140年前与最密切相关的流感谱系发生了分化，包括北美禽流感（PB 1、HA、NA、MP和NS-B）和欧亚谱系（PB 2），以及马的H3 N8谱系（PA、NP和NS-A）。H4、H6和N8亚型的血凝素和神经氨酸酶基因片段的系统发育分析揭示了最近美洲北方和南方半球病毒之间的引入和重配。值得注意的是，尽管最近有血凝素和神经氨酸酶亚型引入的证据，但这些甲型流感病毒内部基因群的系统发育组成保持不变。考虑到目前研究的时间延长和采样物种的数量，以及以前可用数据的缺乏，我们的研究结果有助于更好地了解南美洲流感病毒的生态和进化。
Wild aquatic birds are the major reservoir of influenza A virus. Cloacal swabs and feces samples (n = 6595) were collected from 62 bird species in Argentina from 2006 to 2016 and screened for influenza A virus. Full genome sequencing of 15 influenza isolates from 6 waterfowl species revealed subtypes combinations that were previously described in South America (H1N1, H4N2, H4N6 (n = 3), H5N3, H6N2 (n = 4), and H10N7 (n = 2)), and new ones not previously identified in the region (H4N8, H7N7 and H7N9). Notably, the internal gene segments of all 15 Argentine isolates belonged to the South American lineage, showing a divergent evolution of these viruses in the Southern Hemisphere. Time-scaled phylogenies indicated that South American gene segments diverged between ~ 30 and ~ 140 years ago from the most closely related influenza lineages, which include the avian North American (PB1, HA, NA, MP, and NS-B) and Eurasian lineage (PB2), and the equine H3N8 lineage (PA, NP, and NS-A). Phylogenetic analyses of the hemagglutinin and neuraminidase gene segments of the H4, H6, and N8 subtypes revealed recent introductions and reassortment between viruses from the Northern and Southern Hemispheres in the Americas. Remarkably and despite evidence of recent hemagglutinin and neuraminidase subtype introductions, the phylogenetic composition of internal gene constellation of these influenza A viruses has remained unchanged. Considering the extended time and the number of sampled species of the current study, and the paucity of previously available data, our results contribute to a better understanding of the ecology and evolution of influenza virus in South America.
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影响因子：
5.4
作者：
Liang L;Deng G;Shi J;Wang S;Zhang Q;Kong H;Gu C;Guan Y;Suzuki Y;Li Y;Jiang Y;Tian G;Liu L;Li C;Chen H
通讯作者：
Chen H
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影响因子：
2.7
作者：
Marjuki, Henju;Scholtissek, Christoph;Franks, John;Negovetich, Nicholas J.;Aldridge, Jerry R.;Salomon, Rachelle;Finkelstein, David;Webster, Robert G.
通讯作者：
Webster, Robert G.
DOI：
10.1073/pnas.0507415102
发表时间：
2005-12-20
影响因子：
11.1
作者：
Gabriel, G;Dauber, B;Stech, J
通讯作者：
Stech, J
影响因子：
5.4
作者：
Hurt AC;Su YCF;Aban M;Peck H;Lau H;Baas C;Deng YM;Spirason N;Ellström P;Hernandez J;Olsen B;Barr IG;Vijaykrishna D;Gonzalez-Acuna D
通讯作者：
Gonzalez-Acuna D
影响因子：
4.9
作者：
Abed, Y;Goyette, N;Boivin, G
通讯作者：
Boivin, G