MicroRNA-203 contributes to skin re-epithelialization.
MicroRNA-203 contributes to skin re-epithelialization.
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DOI：
10.1038/cddis.2012.174
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发表时间：
2012-11-29
影响因子：
9
通讯作者：
Candi, E.
中科院分区：
文献类型：
--
作者：
Viticchie, G.;Lena, A. M.;Cianfarani, F.;Odorisio, T.;Annicchiarico-Petruzzelli, M.;Melino, G.;Candi, E.
角质形成细胞的增殖和迁移是伤口愈合过程中表皮快速闭合的关键步骤，但参与这种细胞反应的分子机制仍有待完全阐明。在这里，通过原位杂交，我们表征了小鼠表皮中诱导伤口后miR-203的表达模式，表明其表达在“迁移舌”的高度增殖的角质形成细胞中下调，而在伤口外皮肤的分化细胞中强烈表达。此外，在新生小鼠背部皮肤中皮下注射miR-203在体内加强了miR-203表达与两种新靶mRNA：RAN和RAPH 1之间的负相关性。我们的数据表明，miR-203通过控制负责角质形成细胞增殖和迁移的靶蛋白的表达，在伤口再上皮化和受损皮肤的表皮稳态重建中发挥特定作用。
Keratinocyte proliferation and migration are crucial steps for the rapid closure of the epidermis during wound healing, but the molecular mechanisms involved in this cellular response remain to be completely elucidated. Here, by in situ hybridization we characterize the expression pattern of miR-203 after the induction of wound in mouse epidermis, showing that its expression is downregulated in the highly proliferating keratinocytes of the ‘migrating tongue', whereas it is strongly expressed in the differentiating cells of the skin outside the wound. Furthermore, subcutaneous injections of antagomiR-203 in new born mice dorsal skin strengthened, in vivo, the inverse correlation between miR-203 expression and two new target mRNAs: RAN and RAPH1. Our data suggest that miR-203, by controlling the expression of target proteins that are responsible for both keratinocyte proliferation and migration, exerts a specific role in wound re-epithelialization and epidermal homeostasis re-establishment of injured skin.
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影响因子：
9
作者：
通讯作者：
--
影响因子：
6.5
作者：
Langton, Abigail K.;Herrick, Sarah E.;Headon, Denis J.
通讯作者：
Headon, Denis J.
影响因子：
6
作者：
Cianfarani, Francesca;Zambruno, Giovanna;Odorisio, Teresa
通讯作者：
Odorisio, Teresa
DOI：
10.1073/pnas.1112257109
发表时间：
2012-01-24
影响因子：
11.1
作者：
Cervo, Pia Rivetti di Val;Lena, Anna Maria;Melino, Gerry
通讯作者：
Melino, Gerry
影响因子：
4
作者：
Browne, Gareth;Cipollone, Rita;Melino, Gerry
通讯作者：
Melino, Gerry