Deformability limits of Plasmodium falciparum-infected red blood cells.
Deformability limits of Plasmodium falciparum-infected red blood cells.
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DOI：
10.1111/j.1462-5822.2009.01334.x
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发表时间：
2009-09
影响因子：
3.4
通讯作者：
Rathod PK
中科院分区：
文献类型：
--
作者：
Herricks T;Antia M;Rathod PK
脾脏过滤感染的红细胞（RBC）可能有助于先天免疫和疟疾感染的可变结果。我们表明，个别红细胞的过滤性很好地预测的最小圆柱直径（MCD），这是从红细胞的表面积和体积计算。MCD描述了细胞可以通过而不增加其表面积的最小直径管或最小孔。开发了一种微流体装置来测量来自数千个个体感染的RBC（IRBC）和未感染的RBC（URBC）的MCD。平均MCD的变化在恶性疟原虫的血液阶段的周期进行了跟踪的细胞贴壁菌株ITG和knobless株Dd2。IRBC和URBC的MCD值对脾脏如何清除IRBC提出了几个新的有趣的见解：一些早期的环状IRBC，而不仅仅是晚期的环状IRBC，可能对过滤非常敏感。此外，多节寄生虫可能限制表面积扩张，从而赋予IRBC高MCD。最后，与IRBC一起培养的URBC显示出更高的表面积损失，这使得它们比幼稚URBC更容易过滤。这些发现提出了重要的基本问题，疟疾感染和代谢过程的变量病理影响IRBC的体积和表面积。
Splenic filtration of infected red blood cells (RBCs) may contribute to innate immunity and variable outcomes of malaria infections. We show that filterability of individual RBCs is well predicted by the minimum cylindrical diameter (MCD) which is calculated from a RBC's surface area and volume. The MCD describes the smallest diameter tube or smallest pore that a cell may fit through without increasing its surface area. A microfluidic device was developed to measure the MCD from thousands of individual infected RBCs (IRBCs) and uninfected RBCs (URBCs). Average MCD changes during the blood-stage cycle of Plasmodium falciparum were tracked for the cytoadherent strain ITG and the knobless strain Dd2. The MCD values for IRBCs and URBCs raise several new intriguing insights into how the spleen may remove IRBCs: some early-stage ring-IRBCs, and not just late-stage schizont-IRBCs, may be highly susceptible to filtration. In addition, knobby parasites may limit surface area expansions and thus confer high MCDs on IRBCs. Finally, URBCs, in culture with IRBCs, show higher surface area loss which makes them more susceptible to filtration than naive URBCs. These findings raise important basic questions about the variable pathology of malaria infections and metabolic process that affect volume and surface area of IRBCs.
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DOI：
10.1073/pnas.0711067105
发表时间：
2008-02-19
影响因子：
11.1
作者：
Elliott, David A.;McIntosh, Michael T.;Joiner, Keith A.
通讯作者：
Joiner, Keith A.
影响因子：
20.3
作者：
Douki, JBL;Sterkers, Y;Gysin, J
通讯作者：
Gysin, J
影响因子：
3
作者：
Clark IA;Budd AC;Hsue G;Haymore BR;Joyce AJ;Thorner R;Krause PJ
通讯作者：
Krause PJ
DOI：
10.1073/pnas.93.8.3497
发表时间：
1996-04-16
影响因子：
11.1
作者：
Baruch, DI;Gormley, JA;Pasloske, BL
通讯作者：
Pasloske, BL
DOI：
10.4269/ajtmh.1997.57.507
发表时间：
1997-11-01
影响因子：
3.3
作者：
Dondorp, AM;Angus, BJ;Vreeken, J
通讯作者：
Vreeken, J