Myocardial substrate and route of administration determine acute cardiac retention and lung bio-distribution of cardiosphere-derived cells.
Myocardial substrate and route of administration determine acute cardiac retention and lung bio-distribution of cardiosphere-derived cells.
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DOI：
10.1007/s12350-011-9369-9
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发表时间：
2011-05
影响因子：
2.4
通讯作者：
Abraham, M. Roselle
中科院分区：
文献类型：
--
作者：
Bonios, Michael;Terrovitis, John;Chang, Connie Y.;Engles, James M.;Higuchi, Takahiro;Lautamaeki, Riikka;Yu, Jianhua;Fox, James;Pomper, Martin;Wahl, Richard L.;Tsui, Benjamin M.;O'Rourke, Brian;Bengel, Frank M.;Marban, Eduardo;Abraham, M. Roselle
移植后急性心肌滞留和心球源性细胞（CDC）的肺生物分布的定量对于改善植入是重要的。我们研究了4组（n = 25）大鼠的急性（1小时）心/肺潴留（正常NL、急性缺血再灌注AI-RM、急性永久性结扎PL和缺血再灌注CI-R所致慢性梗死），1组使用冠状动脉内给药急性缺血再灌注组（AI-RC）和静脉注射组（AI-RV）。NL、AI-RM、CI-R和A-IRC组的心脏潴留相似（13.6% ± 2.3% vs 12.0% ± 3.9% vs 9.9 ± 2.8 vs 15.4% ± 5.5%; P = NS），但PL动物的心脏潴留更高（22.9% ± 5.2%; P <0.05）。与PL（28.5% ± 5.9%）、CI-R（20.2% ± 9.3%）和A-IRC（19.9% ± 5.6%）动物相比，NL和AI-RM组（43.3% ± 5.6%和39.9% ± 9.3%）的低心脏潴留与显著更高的肺活动相关（P <0.05 vs AI-RM和NL）。静脉内CDC给药后肺活性最高（55.1% ± 9.3%，P <0.001），并与极低的心脏潴留（0.8% ± 1.06%）相关。双光子显微镜观察表明，心肌内注射后，CDC通过冠状静脉逃逸到肺部。急性心滞留和肺生物分布随心肌基质和注射途径而变化。心肌内注射的CDC通过冠状静脉逃逸到肺中，这种作用在灌注心肌中更明显。
Quantification of acute myocardial retention and lung bio-distribution of cardiosphere-derived cells (CDCs) following transplantation is important to improve engraftment. We studied acute(1 hour) cardiac/lung retention in 4 groups (n = 25) of rats (normal—NL, acute ischemia-reperfusion—AI-RM, acute permanent ligation—PL, and chronic infarct by ischemia-reperfusion—CI-R) using intra-myocardial delivery, 1 group using intracoronary delivery (acute ischemia-reperfusion, AI-RC, n = 5) and 1 group using intravenous delivery (acute ischemia-reperfusion, AI-RV, n = 5) of CDCs by PET. Cardiac retention was similar in the NL, AI-RM, CI-R, and A-IRC groups (13.6% ± 2.3% vs 12.0% ± 3.9% vs 9.9 ± 2.8 vs 15.4% ± 5.5%; P = NS), but higher in PL animals (22.9% ± 5.2%; P < .05). Low cardiac retention was associated with significantly higher lung activity in NL and AI-RM groups (43.3% ± 5.6% and 39.9% ± 9.3%), compared to PL (28.5% ± 5.9%), CI-R (20.2% ± 9.3%), and A-IRC (19.9% ± 5.6%) animals (P < .05 vs AI-RM and NL). Lung activity was highest following intravenous CDC delivery (55.1% ± 9.3%, P < .001) and was associated with very low cardiac retention (0.8% ± 1.06%). Two-photon microscopy indicated that CDCs escaped to the lungs via the coronary veins following intra-myocardial injection. Acute cardiac retention and lung bio-distribution vary with the myocardial substrate and injection route. Intra-myocardially injected CDCs escape into the lungs via coronary veins, an effect that is more pronounced in perfused myocardium.
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影响因子：
3.7
作者：
Davis DR;Zhang Y;Smith RR;Cheng K;Terrovitis J;Malliaras K;Li TS;White A;Makkar R;Marbán E
通讯作者：
Marbán E
影响因子：
24
作者：
Hare, Joshua M.;Traverse, Jay H.;Henry, Timothy D.;Dib, Nabil;Strumpf, Robert K.;Schulman, Steven P.;Gerstenblith, Gary;DeMaria, Anthony N.;Denktas, Ali E.;Gammon, Roger S.;Hermiller, James B., Jr.;Reisman, Mark A.;Schaer, Gary L.;Sherman, Warren
通讯作者：
Sherman, Warren
影响因子：
24
作者：
Terrovitis, John;Lautamaeki, Riikka;Bonios, Michael;Fox, James;Engles, James M.;Yu, Jianhua;Leppo, Michelle K.;Pomper, Martin G.;Wahl, Richard L.;Seidel, Jurgen;Tsui, Benjamin M.;Bengel, Frank M.;Abraham, M. Roselle;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
37.8
作者：
Hofmann, M;Wollert, KC;Drexler, H
通讯作者：
Drexler, H
DOI：
10.1016/j.jtcvs.2006.05.034
发表时间：
2006-09-01
影响因子：
6
作者：
Teng, Carolyn J.;Luo, Jun;Shum-Tim, Dominique
通讯作者：
Shum-Tim, Dominique