Noninvasive quantification and optimization of acute cell retention by in vivo positron emission tomography after intramyocardial cardiac-derived stem cell delivery.
Noninvasive quantification and optimization of acute cell retention by in vivo positron emission tomography after intramyocardial cardiac-derived stem cell delivery.
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DOI：
10.1016/j.jacc.2009.04.097
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发表时间：
2009-10-20
影响因子：
24
通讯作者：
Marban, Eduardo
中科院分区：
文献类型：
--
作者：
Terrovitis, John;Lautamaeki, Riikka;Bonios, Michael;Fox, James;Engles, James M.;Yu, Jianhua;Leppo, Michelle K.;Pomper, Martin G.;Wahl, Richard L.;Seidel, Jurgen;Tsui, Benjamin M.;Bengel, Frank M.;Abraham, M. Roselle;Marban, Eduardo
目的：应用正电子发射断层扫描(PET)对心脏干细胞(CDCs)在急性心肌中的滞留进行量化，并评价不同的输送方法。干细胞移植用于心脏再生的成功部分受制于移植细胞的低保留率/植入率。一种临床上适用的准确量化细胞滞留的方法将使细胞输送最优化。取同基因雄性Wistar京都(WK)大鼠的CDC，用18FDG标记，注入永久性左冠状动脉结扎后的WK雌性大鼠心肌缺血区。观察纤维蛋白胶、心动过缓(腺苷)和心脏骤停的效果。18FDGPET用于细胞滞留的定量。对男性特异的SRY基因进行定量聚合酶链式反应以验证PET结果。产后1小时悬浮于PBS中的细胞的心肌滞留率分别为17.6±11.5%和17.8±7.3%。在心脏骤停后立即注射CDCs，滞留率增加到75.6±18.6%。腺苷使心室率减慢，CDC滞留率增加一倍(35.4±5.3%)。在心外膜注射部位应用纤维蛋白胶后，CDC滞留也有类似的增加(37.5±8.2%)。聚合酶链式反应显示，纤维蛋白胶组3周细胞植入率显著增加(22.1±18.6%，PBS组5.3±3.1%)。活体正电子发射计算机断层扫描可准确测量心肌内给药后早期CDC滞留。用纤维蛋白胶封闭注射部位或用腺苷降低心室率可能是改善心脏跳动中干细胞植入的临床可翻译方法。
To quantify acute myocardial retention of cardiac-derived stem cells (CDCs) and evaluate different delivery methods using Positron Emission Tomography (PET). Success of stem cell transplantation for cardiac regeneration is partially limited by low retention/engraftment of the delivered cells. A clinically applicable method for accurate quantification of cell retention would enable optimization of cell delivery. CDCs derived from syngeneic, male Wistar Kyoto (WK) rats were labeled with 18FDG and injected intramyocardially into the ischemic region of female WK rats following permanent left coronary artery ligation. The effects of fibrin glue, bradycardia (adenosine) and cardiac arrest were examined. 18FDG PET was performed for quantification of cell retention. Quantitative PCR for the male-specific SRY gene was performed to validate the PET results. Myocardial retention of cells suspended in PBS 1 hr after delivery was 17.6±11.5% by PCR and 17.8±7.3% by PET. When CDCs were injected immediately following induction of cardiac arrest, retention was increased to 75.6±18.6%. Adenosine slowed the ventricular rate and doubled CDC retention (35.4±5.3%). A similar increase in CDC retention was observed following epicardial application of fibrin glue at the injection site (37.5±8.2%). PCR revealed a significant increase in 3 week cell engraftment in the fibrin glue animals (22.1±18.6% vs 5.3±3.1%, for fibrin glue and PBS respectively). In vivo PET permits accurate measurement of CDC retention early after intramyocardial delivery. Sealing injection sites with fibrin glue or lowering ventricular rate by adenosine may be clinically translatable methods for improving stem cell engraftment in a beating heart.
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DOI：
10.1007/s00259-005-1898-5
发表时间：
2005-12-01
影响因子：
9.1
作者：
Bengel, Frank M.;Schachinger, Volker;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
影响因子：
37.8
作者：
Cao, F;Lin, S;Wu, JC
通讯作者：
Wu, JC
影响因子：
37.8
作者：
Hofmann, M;Wollert, KC;Drexler, H
通讯作者：
Drexler, H
DOI：
10.1016/s1091-255x(98)80071-1
发表时间：
1998-07-01
期刊：
Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
影响因子：
--
作者：
Hayashi, M;Martinez, O M;Esquivel, C O
通讯作者：
Esquivel, C O
影响因子：
37.8
作者：
Kraitchman, DL;Tatsumi, M;Bulte, JWM
通讯作者：
Bulte, JWM