Analysis of the Heterogeneity of CD4(+)CD25(+) T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice.
Analysis of the Heterogeneity of CD4(+)CD25(+) T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice.
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DOI：
10.1155/2020/3184190
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发表时间：
2020
影响因子：
4.1
通讯作者：
Yao X
中科院分区：
文献类型：
--
作者：
Zhang T;Duan F;Su D;Ma L;Yang J;Shi B;He X;Ma R;Sun S;Yao X
研究4T1 BALB/c小鼠乳腺肿瘤组织、肺转移组织和脾脏中CD4+CD25+ T细胞受体β-链互补决定区3 （TCR β CDR3）谱的同质性和异质性。我们采用高通量测序分析了肿瘤组织、肺转移组织和脾脏中CD4+CD25+ TCR β CDR3基因库的特征和变化。乳腺肿瘤组织中CD4+CD25+ TCR β CDR3基因库的多样性与肺转移组织相似，低于脾组织。乳腺肿瘤组织和肺转移组织中高频CDR3序列和中频CDR3序列数量多于脾脏。在乳腺肿瘤组织和肺转移组织中，独特产性CDR3序列的比例明显大于脾脏。CDR3谱库的多样性和频率在乳腺肿瘤和肺转移组织中保持同质性，在脾脏中表现出较大的异质性，提示乳腺组织和肺转移组织具有与肿瘤微环境相关的CD4+CD25+ T细胞特征。然而，重叠CDR3序列的数量和特征表明，在肿瘤和循环免疫系统中存在一些不同的CD4+CD25+ T细胞。该研究可用于进一步探索CDR3基因库的特征，确定乳腺癌微环境中CD4+CD25+ T细胞的来源。
To study the homogeneity and heterogeneity of CD4+CD25+ T cells receptor β-chain complementarity determining region 3 (TCR β CDR3) repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mice. We used high-throughput sequencing to analyze the characteristics and changes of CD4+CD25+ TCR β CDR3 repertoires among tumor tissues, lung metastatic tissues, and spleens. The diversity of the CD4+CD25+ TCR β CDR3 repertoires in breast tumor tissue was similar to that of lung metastatic tissues and less pronounced than that of spleen tissues. Breast tumor tissues and lung metastatic tissues had a greater number of high-frequency CDR3 sequences and intermediate-frequency CDR3 sequences than those of spleens. The proportion of unique productive CDR3 sequences in breast tumor tissues and lung metastatic tissues was significantly greater than that in the spleens. The diversity and frequency of the CDR3 repertoires remained homogeneous in breast tumors and lung metastatic tissues and showed great heterogeneity in the spleens, which suggested that the breast tissues and lung metastatic tissues have characteristics of CD4+CD25+ T cells that relate to the tumor microenvironment. However, the number and characteristics of overlapping CDR3 sequences suggested that there were some different CD4+CD25+ T cells in tumors and in the circulatory immune system. The study may be used to further explore the characteristics of the CDR3 repertoires and determine the source of the CD4+CD25+ T cells in the breast cancer microenvironment.
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影响因子：
11.2
作者：
Olkhanud PB;Baatar D;Bodogai M;Hakim F;Gress R;Anderson RL;Deng J;Xu M;Briest S;Biragyn A
通讯作者：
Biragyn A
影响因子：
--
作者：
Lefranc, Marie-Paule
通讯作者：
Lefranc, Marie-Paule
影响因子：
30.8
作者：
Li, Bo;Li, Taiwen;Liu, X. Shirley
通讯作者：
Liu, X. Shirley
影响因子：
5.4
作者：
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通讯作者：
Silva, Joao S.
影响因子：
7.3
作者：
Chen, Zengchao;Zhang, Chaoting;Ke, Yang
通讯作者：
Ke, Yang