Quantitative mapping and minimization of super-resolution optical imaging artifacts.
Quantitative mapping and minimization of super-resolution optical imaging artifacts.
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DOI:
10.1038/nmeth.4605
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发表时间:
2018-04
期刊:
影响因子:
48
通讯作者:
Henriques R
中科院分区:
文献类型:
--
作者:
Culley S;Albrecht D;Jacobs C;Pereira PM;Leterrier C;Mercer J;Henriques R
Most super-resolution microscopy techniques depend on steps that can contribute to the formation of image artefacts, leading to misinterpretation of biological information. We present NanoJ-SQUIRREL, an ImageJ-based analytical approach that provides quantitative assessment of super-resolution image quality, capable of guiding researchers in optimising imaging parameters. By comparing diffraction-limited images and super-resolution equivalents of the same acquisition volume, this approach generates a quality score and quantitative map of super-resolution defects.
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