DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria.
DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria.
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DOI：
10.1038/s41467-017-01365-y
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发表时间：
2017-11-01
影响因子：
16.6
通讯作者：
Doherty AJ
中科院分区：
文献类型：
--
作者：
Płociński P;Brissett NC;Bianchi J;Brzostek A;Korycka-Machała M;Dziembowski A;Dziadek J;Doherty AJ
脯氨酸连接酶D是一种保守的DNA修复装置，在稳定期处理DNA双链断裂。邻苯二甲酸连接酶C（LigC）复合物也共存于许多细菌物种中，但其功能尚不清楚。在这里，我们表明，LigC复合物与核心BER酶在体内相互作用，并证明这些因素共同构成一个切除修复装置能够修复受损的碱基和脱碱基位点。聚合酶组分含有保守的C-末端结构环，优先结合并用RNA填充短缺口的DNA中间体，并且LigC连接所得切口以完成修复。LigC复合物的组分，如LigD，在进入稳定期时表达，缺乏这些途径中的任一种的细胞表现出对氧化性基因毒素的敏感性增加。总之，这些发现确立了LigC复合物直接参与切除修复途径，该途径在稳定期期间用核糖核苷酸修复DNA损伤。连接酶D是一种保守的DNA修复蛋白复合物，在静止期原核生物中修复双链断裂。在这里，作者表明，orthoglycoprotein连接酶C在稳定期的碱基切除修复中发挥作用。
Prokaryotic Ligase D is a conserved DNA repair apparatus processing DNA double-strand breaks in stationary phase. An orthologous Ligase C (LigC) complex also co-exists in many bacterial species but its function is unknown. Here we show that the LigC complex interacts with core BER enzymes in vivo and demonstrate that together these factors constitute an excision repair apparatus capable of repairing damaged bases and abasic sites. The polymerase component, which contains a conserved C-terminal structural loop, preferentially binds to and fills-in short gapped DNA intermediates with RNA and LigC ligates the resulting nicks to complete repair. Components of the LigC complex, like LigD, are expressed upon entry into stationary phase and cells lacking either of these pathways exhibit increased sensitivity to oxidising genotoxins. Together, these findings establish that the LigC complex is directly involved in an excision repair pathway(s) that repairs DNA damage with ribonucleotides during stationary phase. Ligase D is a conserved DNA repair protein complex that repairs double-strand breaks in stationary phase prokaryotes. Here the authors show that orthologous Ligase C has a role in base excision repair during stationary phase.
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影响因子：
14.9
作者：
Guilliam TA;Keen BA;Brissett NC;Doherty AJ
通讯作者：
Doherty AJ
影响因子：
14.9
作者：
de Ory A;Nagler K;Carrasco B;Raguse M;Zafra O;Moeller R;de Vega M
通讯作者：
de Vega M
影响因子：
16.8
作者：
Kent, Tatiana;Chandramouly, Gurushankar;McDevitt, Shane Michael;Ozdemir, Ahmet Y.;Pomerantz, Richard T.
通讯作者：
Pomerantz, Richard T.
影响因子：
8.8
作者：
Brissett NC;Martin MJ;Bartlett EJ;Bianchi J;Blanco L;Doherty AJ
通讯作者：
Doherty AJ
影响因子：
3.2
作者：
Jacewicz, Agata;Shuman, Stewart
通讯作者：
Shuman, Stewart