Genome-wide identification and functional characterization of the Camelina sativa WRKY gene family in response to abiotic stress.
Genome-wide identification and functional characterization of the Camelina sativa WRKY gene family in response to abiotic stress.
复制标题
亚麻荠WRKY基因家族响应非生物胁迫的全基因组鉴定和功能表征
DOI：
10.1186/s12864-020-07189-3
复制
发表时间：
2020-11-11
期刊：
影响因子：
4.4
通讯作者：
Li R
中科院分区：
文献类型：
--
作者：
Song Y;Cui H;Shi Y;Xue J;Ji C;Zhang C;Yuan L;Li R
背景
WRKY 转录因子是参与植物多种生物过程和胁迫反应的调节因子超家族。然而，人们对亚麻荠（Camelina sativa）WRKY家族的了解有限，亚麻荠是一种重要的十字花科油料作物，对各种胁迫具有很强的耐受性。在这里，对 WRKY 蛋白进行全基因组表征，以检查其基因结构、系统发育、表达、保守基序组织和功能注释，以确定介导亚麻荠抗逆性调节的候选 WRKY。
结果
共鉴定出由染色体上不均匀分布的224个基因位点编码的242个CsWRKY蛋白，并通过系统发育分析根据其WRKY结构域和锌指基序将它们分为三类。 15 个 CsWRKY 基因位点产生 33 个剪接变体。鉴定出直系同源 WRKY 基因对，在苜蓿和拟南芥基因组中分别有 173 对，在苜蓿和欧洲油菜基因组中分别有 282 对。总共观察到 137 个节段重复事件，但亚麻荠基因组中没有串联重复。检查了 10 个主要保守基序，其中 WRKYGQK 是最保守的，并且许多 CsWRKY 中存在多种变体。表达分析显示，CsWRKY 基因的组成型表达增加了 50%，并且其中一组表现出组织特异性表达。值得注意的是，在寒冷、盐和干旱胁迫下，11个CsWRKY基因在幼苗中表现出显着的表达变化，表现出响应胁迫的优先诱导表达模式。
结论
本文描述了对 CsWRKY 基因家族及其在 12 种组织和几种应激条件下的表达谱的详细分析。片段重复是该基因家族广泛扩展的主要力量，CsWRKY 蛋白在进化过程中产生了强大的纯化压力。 CsWRKY 蛋白在植物发育中发挥重要作用，在不同组织中具有不同的功能。特殊的是，11 个 CsWRKY，特别是 5 个选择性剪接亚型，被发现可能是介导植物对各种胁迫反应的关键角色。总体而言，我们的结果为了解 CsWRKY 的作用以及 CsWRKY 调节十字花科作物的高抗逆性以及抗逆品种开发的精确机制奠定了基础。
Background
WRKY transcription factors are a superfamily of regulators involved in diverse biological processes and stress responses in plants. However, there is limited knowledge about the WRKY family in camelina (Camelina sativa), an important Brassicaceae oil crop with strong tolerance for various stresses. Here, a genome-wide characterization of WRKY proteins is performed to examine their gene structures, phylogenetics, expression, conserved motif organizations, and functional annotation to identify candidate WRKYs that mediate stress resistance regulation in camelinas.
Results
A total of 242 CsWRKY proteins encoded by 224 gene loci distributed unevenly over the chromosomes were identified, and they were classified into three groups by phylogenetic analysis according to their WRKY domains and zinc finger motifs. The 15 CsWRKY gene loci generated 33 spliced variants. Orthologous WRKY gene pairs were identified, with 173 pairs in the C. sativa and Arabidopsis genomes as well as 282 pairs in the C. sativa and B. napus genomes, respectively. A total of 137 segmental duplication events were observed, but there was no tandem duplication in the camelina genome. Ten major conserved motifs were examined, with WRKYGQK being the most conserved, and several variants were present in many CsWRKYs. Expression analysis revealed that 50% more CsWRKY genes were expressed constitutively, and a set of them displayed tissue-specific expression. Notably, 11 CsWRKY genes exhibited significant expression changes in seedlings under cold, salt, and drought stresses, showing a preferentially inducible expression pattern in response to the stress.
Conclusions
The present article describes a detailed analysis of the CsWRKY gene family and its expression profiles in 12 tissues and under several stress conditions. Segmental duplication is the major force underlying the broad expansion of this gene family, and a strong purifying pressure occurred for CsWRKY proteins during their evolution. CsWRKY proteins play important roles in plant development, with differential functions in different tissues. Exceptionally, eleven CsWRKYs, particularly five alternative spliced isoforms, were found to be the possible key players in mediating plant responses to various stresses. Overall, our results provide a foundation for understanding the roles of CsWRKYs and the precise mechanism through which CsWRKYs regulate high stress resistance as well as the development of stress tolerance cultivars among Cruciferae crops.
登录
查看更多内容
影响因子：
4.4
作者：
Liang C;Liu X;Yiu SM;Lim BL
通讯作者：
Lim BL
多重胁迫下甘蓝型油菜WRKY转录因子的全基因组鉴定及表达分析
影响因子：
3.7
作者：
He, Yajun;Mao, Shaoshuai;Qian, Wei
通讯作者：
Qian, Wei
乌苏里梨 bHLH1 转录因子的过表达可增强其耐冷性并增加应激反应基因的表达
影响因子：
5.6
作者：
Jin C;Huang XS;Li KQ;Yin H;Li LT;Yao ZH;Zhang SL
通讯作者：
Zhang SL
芝麻基因组中WRKY基因家族的全基因组分析以及参与非生物胁迫响应的WRKY基因的鉴定
影响因子：
5.3
作者：
Li D;Liu P;Yu J;Wang L;Dossa K;Zhang Y;Zhou R;Wei X;Zhang X
通讯作者：
Zhang X
影响因子：
6.3
作者：
Abdullah HM;Akbari P;Paulose B;Schnell D;Qi W;Park Y;Pareek A;Dhankher OP
通讯作者：
Dhankher OP