Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses.
Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses.
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芝麻基因组中WRKY基因家族的全基因组分析以及参与非生物胁迫响应的WRKY基因的鉴定
DOI：
10.1186/s12870-017-1099-y
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发表时间：
2017-09-11
影响因子：
5.3
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Li D;Liu P;Yu J;Wang L;Dossa K;Zhang Y;Zhou R;Wei X;Zhang X
芝麻（Sesamum indicum L.）是世界上最重要的油料作物之一。然而，它一般易受非生物胁迫的影响，尤其易受水涝和干旱胁迫的影响。芝麻抗非生物胁迫的分子机制尚未阐明。WRKY结构域转录因子在植物的生长发育和逆境应答中起着重要作用。然而，对芝麻WRKY基因的数量、位置、结构、分子遗传学和表达知之甚少。我们对芝麻中的WRKY基因家族进行了全面的研究，并鉴定了71个SiWRKY。总共有65个基因被映射到芝麻基因组内的15个连锁群。利用近缘物种拟南芥（Arabidopsis thaliana）进行系统发育分析，以研究芝麻WRKY基因的进化。WRKY基因的组织表达谱表明，6个SiWRKY基因在所有器官中高表达，这表明这些基因可能是重要的植物生长和器官发育在芝麻。SiWRKY基因表达模式的分析表明，33和26 SiWRKY强烈响应水涝和干旱胁迫，分别。12 SiWRKY基因的表达的变化，观察在不同的时间后，渍水和干旱处理已经开始，表明芝麻基因的表达模式不同，在响应非生物胁迫。在这项研究中，我们分析了由芝麻基因组编码的WRKY家族转录因子。深入了解了SiWRKY基因的分类、进化和功能，揭示了它们在各种组织中的假定作用。同时研究了不同芝麻品种对非生物胁迫的反应。我们的研究结果提供了一个更好的理解芝麻WRKY基因的结构和功能，并建议操纵这些WRKY可以提高对水涝和干旱的抗性。本文的在线版本（10.1186/s12870-017-1099-y）包含补充材料，可供授权用户使用。
Sesame (Sesamum indicum L.) is one of the world’s most important oil crops. However, it is susceptible to abiotic stresses in general, and to waterlogging and drought stresses in particular. The molecular mechanisms of abiotic stress tolerance in sesame have not yet been elucidated. The WRKY domain transcription factors play significant roles in plant growth, development, and responses to stresses. However, little is known about the number, location, structure, molecular phylogenetics, and expression of the WRKY genes in sesame. We performed a comprehensive study of the WRKY gene family in sesame and identified 71 SiWRKYs. In total, 65 of these genes were mapped to 15 linkage groups within the sesame genome. A phylogenetic analysis was performed using a related species (Arabidopsis thaliana) to investigate the evolution of the sesame WRKY genes. Tissue expression profiles of the WRKY genes demonstrated that six SiWRKY genes were highly expressed in all organs, suggesting that these genes may be important for plant growth and organ development in sesame. Analysis of the SiWRKY gene expression patterns revealed that 33 and 26 SiWRKYs respond strongly to waterlogging and drought stresses, respectively. Changes in the expression of 12 SiWRKY genes were observed at different times after the waterlogging and drought treatments had begun, demonstrating that sesame gene expression patterns vary in response to abiotic stresses. In this study, we analyzed the WRKY family of transcription factors encoded by the sesame genome. Insight was gained into the classification, evolution, and function of the SiWRKY genes, revealing their putative roles in a variety of tissues. Responses to abiotic stresses in different sesame cultivars were also investigated. The results of our study provide a better understanding of the structures and functions of sesame WRKY genes and suggest that manipulating these WRKYs could enhance resistance to waterlogging and drought. The online version of this article (10.1186/s12870-017-1099-y) contains supplementary material, which is available to authorized users.
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影响因子：
3.1
作者：
Huang, Shengxiong;Gao, Yongfeng;Liu, Yongsheng
通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
Diao WP;Snyder JC;Wang SB;Liu JB;Pan BG;Guo GJ;Wei G
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影响因子：
4.4
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