Mating Damages the Cuticle of C. elegans Hermaphrodites
Mating Damages the Cuticle of C. elegans Hermaphrodites
复制标题
交配会损害线虫雌雄同体的角质层
作者：
Gavin C. Woodruff;C. Knauss;T. Maugel;E. Haag
繁殖的寿命成本在多个物种中很常见，并且这种成本可能通过多种机制产生。在秀丽隐杆线虫中，有人认为，雌雄同体因交配而损失的部分寿命是由于交配本身造成的物理损伤造成的。在这里，我们通过扫描电子显微镜检查交配是否损害雌雄同体角质层的表面。研究发现，交配的雌雄同体外阴周围的角质层会发生分层，这种损伤的发生率取决于遗传背景。未交配的雌雄同体几乎没有表现出这种损害，即使在含有潜在研磨颗粒的土壤中培养也是如此。因此，线虫雌雄同体交配的结果是物理角质层损伤。这些实验没有评估角质层损伤对寿命的影响，这种损伤的生物学意义仍不清楚。我们在最近的研究背景下进一步讨论了我们的结果，这些研究将线虫雌雄同体的寿命成本与雄性分泌物联系起来。
Lifespan costs to reproduction are common across multiple species, and such costs could potentially arise through a number of mechanisms. In the nematode Caenorhabditis elegans, it has been suggested that part of the lifespan cost to hermaphrodites from mating results from physical damage owing to the act of copulation itself. Here, we examine whether mating damages the surface of the hermaphrodite cuticle via scanning electron microscopy. It is found that mated hermaphrodites suffered delamination of cuticle layers surrounding the vulva, and that the incidence of such damage depends on genetic background. Unmated hermaphrodites demonstrated almost no such damage, even when cultured in soil with potentially abrasive particles. Thus, a consequence of mating for C. elegans hermaphrodites is physical cuticle damage. These experiments did not assess the consequences of cuticle damage for lifespan, and the biological significance of this damage remains unclear. We further discuss our results within the context of recent studies linking the lifespan cost to mating in C. elegans hermaphrodites to male secretions.
秀丽隐杆线虫雄性长寿的遗传、行为和环境决定因素。
影响因子：
3.3
作者：
Gems,D;Riddle,DL
通讯作者：
Riddle,DL
影响因子：
23.8
作者：
Avila FW;Sirot LK;LaFlamme BA;Rubinstein CD;Wolfner MF
通讯作者：
Wolfner MF