Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing.
Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing.
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DOI：
10.1039/d1ra08610c
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发表时间：
2021-12-13
期刊：
影响因子：
3.9
通讯作者：
Goddard, Nicholas J.
中科院分区：
文献类型：
--
作者：
Gupta, Ruchi;El Sayed, Sameh;Goddard, Nicholas J.
这是一个前所未有的报告，水凝胶光栅与分析物响应染料固定在交替带，其中图案化的染料是其自己的色散元件，以执行光谱。在每个波长下，水凝胶光栅的衍射效率是染料吸光度的函数，而染料吸光度又取决于样品中分析物的浓度。因此，测量水凝胶光栅的衍射光强度的变化以感测分析物。同样地，在两个波长处的衍射强度的比率用于分析物的定量，以减少由光源强度的变化和染料的光漂白引起的误差。通过在激光干涉光刻装置中曝光175 μm厚的可光官能化的聚丙烯酰胺水凝胶膜，产生具有和不具有自由官能团的交替线阵列，来制造15.27 μm节距光栅。释放的官能团与pH敏感的异硫氰酸荧光素反应，以创建光栅测量pH值。在430和475 nm处的水凝胶光栅的衍射光的强度的比率被证明是线性超过4个pH单位，这与传统的吸收光谱法相比，有利的是102个pH单位。这种增加的动态范围是分析物响应染料的pH响应和作为染料吸光度的函数的衍射效率中的相反非线性的抵消的结果。这是一个前所未有的水凝胶光栅与分析物响应染料固定在交替带，其中图案化的染料是它自己的色散元件进行光谱传感的报告。
This is an unprecedented report of hydrogel gratings with an analyte responsive dye immobilised in alternating strips where the patterned dye is its own dispersive element to perform spectroscopy. At each wavelength, the diffraction efficiency of hydrogel gratings is a function of dye absorbance, which in turn is dependent on the concentration of analytes in samples. Thus, changes in intensity of diffracted light of hydrogel gratings were measured for sensing of analytes. Equally, the ratio of diffracted intensities at two wavelengths was used for quantification of analytes to reduce errors caused by variations in intensity of light sources and photobleaching of dyes. 15.27 μm pitch gratings were fabricated by exposing 175 μm thick films of photofunctionalisable poly(acrylamide) hydrogel in a laser interferometric lithography setup, generating an array of alternating lines with and without free functional groups. The freed functional groups were reacted with pH sensitive fluorescein isothiocyanate to create gratings for measurement of pH. The ratio of intensity of diffracted light of hydrogel gratings at 430 and 475 nm was shown to be linear over 4 pH units, which compares favourably with ∼2 pH units for conventional absorption spectroscopy. This increased dynamic range was a result of cancellation of the opposite non-linearities in the pH response of the analyte responsive dye and the diffraction efficiency as a function of dye absorbance. This is an unprecedented report of hydrogel gratings with an analyte responsive dye immobilised in alternating strips where the patterned dye is its own dispersive element to perform spectroscopic sensing.
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影响因子：
4.6
作者：
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