Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes.
Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes.
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DOI：
10.1038/s41598-017-03399-0
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发表时间：
2017-06-13
影响因子：
4.6
通讯作者：
St Leger RJ
中科院分区：
文献类型：
--
作者：
Bilgo E;Lovett B;Fang W;Bende N;King GF;Diabate A;St Leger RJ
疟疾控制工作要继续取得成功，就需要开发、研究和实施避免杀虫剂抗药性的新技术。我们以前证明，真菌病原体可以提供一个有效的灭蚊或杀疟疾生物分子的传递系统。在这里，我们比较了来自节肢动物捕食者的编码昆虫特异性钠，钾和钙通道阻滞剂的基因，以提高绿僵菌对野生捕获的，抗草甘膦的按蚊的功效。在血淋巴特异性启动子控制下表达的毒素在孢子剂量低至每只蚊子一个分生孢子时增加了真菌对蚊子的致死性。研究了最有效的药物之一EPA批准的Hybrid（Ca++/K+通道阻滞剂）的致死前效应。这些包括减少吸血行为，几乎100%的昆虫感染了~6个孢子，在感染后5天内无法传播疟疾，超过了世界卫生组织成功的病媒控制剂的阈值。此外，共表达杂合毒素和AaIT（Na+通道阻断剂）的重组菌株产生协同效应，与单一毒素菌株相比，在5天内需要少45%的孢子才能杀死一半的蚊子。我们的研究结果确定了具有不同作用模式的毒素库，这些毒素提高了昆虫病原体作为与现有基于杀虫剂的控制方法兼容的技术的效用。
The continued success of malaria control efforts requires the development, study and implementation of new technologies that circumvent insecticide resistance. We previously demonstrated that fungal pathogens can provide an effective delivery system for mosquitocidal or malariacidal biomolecules. Here we compared genes from arthropod predators encoding insect specific sodium, potassium and calcium channel blockers for their ability to improve the efficacy of Metarhizium against wild-caught, insecticide-resistant anophelines. Toxins expressed under control of a hemolymph-specific promoter increased fungal lethality to mosquitoes at spore dosages as low as one conidium per mosquito. One of the most potent, the EPA approved Hybrid (Ca++/K+ channel blocker), was studied for pre-lethal effects. These included reduced blood feeding behavior, with almost 100% of insects infected with ~6 spores unable to transmit malaria within 5 days post-infection, surpassing the World Health Organization threshold for successful vector control agents. Furthermore, recombinant strains co-expressing Hybrid toxin and AaIT (Na+ channel blocker) produced synergistic effects, requiring 45% fewer spores to kill half of the mosquitoes in 5 days as single toxin strains. Our results identify a repertoire of toxins with different modes of action that improve the utility of entomopathogens as a technology that is compatible with existing insecticide-based control methods.
影响因子：
3
作者：
Konrad BP;Lindstrom M;Gumpinger A;Zhu J;Coombs D
通讯作者：
Coombs D