Developmental neural activity requires neuron-astrocyte interactions.
Developmental neural activity requires neuron-astrocyte interactions.
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DOI：
10.1002/dneu.22870
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发表时间：
2022-04
影响因子：
3
通讯作者：
Akin, Orkun
中科院分区：
文献类型：
--
作者：
Bajar, Bryce T.;Phi, Nguyen T.;Randhawa, Harpreet;Akin, Orkun
发育性神经活动是神经回路组装的共同特征。虽然胶质细胞在突触发育中的作用已经确立，但神经元-胶质细胞相互作用对发育活动的贡献在很大程度上仍未被探索。在这里，我们展示了在果蝇突触发生过程中，星形胶质细胞是发育活动所必需的。利用广域荧光和双光子成像，我们证明了中枢神经系统的胶质细胞参与发育活动，并具有细胞内钙动力学的特定模式。基因消融星形胶质细胞，而不是皮质或包膜胶质细胞，会导致神经元活动的严重减弱。同样，神经元活动的抑制会导致星形胶质细胞钙动力学的丧失。通过改变这些动力学，我们表明星形细胞钙循环可以影响神经元的活动，但本身并不是必需的。综上所述，我们的结果表明，星形胶质细胞除了在突触的结构成熟中起着公认的作用外，在突触发育过程中也是突触功能所必需的。
Developmental neural activity is a common feature of neural circuit assembly. Although glia have established roles in synapse development, the contribution of neuron-glia interactions to developmental activity remains largely unexplored. Here we show that astrocytes are necessary for developmental activity during synaptogenesis in Drosophila. Using wide-field epifluorescence and two-photon imaging, we show that the glia of the central nervous system participate in developmental activity with type-specific patterns of intracellular calcium dynamics. Genetic ablation of astrocytes, but not of cortex or ensheathing glia, leads to severe attenuation of neuronal activity. Likewise, inhibition of neuronal activity results in the loss of astrocyte calcium dynamics. By altering these dynamics, we show that astrocytic calcium cycles can influence neuronal activity but are not necessary per se. Taken together, our results indicate that, in addition to their recognized role in the structural maturation of synapses, astrocytes are also necessary for the function of synapses during development.
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