Identification and characterization of MT-1X as a novel FHL3-binding partner.
Identification and characterization of MT-1X as a novel FHL3-binding partner.
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MT-1X 作为新型 FHL3 结合伴侣的鉴定和表征。
DOI：
10.1371/journal.pone.0093723
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xu D
中科院分区：
文献类型：
--
作者：
Cai X;Wang J;Huang X;Fu W;Xia W;Zou M;Wang Y;Wang J;Xu D
四个半 LIM 结构域蛋白 3 (FHL3) 是 FHL 蛋白家族的成员，在细胞存活、细胞粘附和信号转导的调节中发挥作用。然而，FHL3的作用机制尚不清楚。本研究的目的是鉴定 FHL3 的新结合伴侣并探索其潜在机制。利用酵母双杂交筛选系统，以FHL3为诱饵，筛选人胎肝cDNA文库中的相互作用蛋白。 Methionine-1X 被鉴定为新型 FHL3 结合伴侣。 FHL3 和全长 MT-1X 之间的相互作用通过酵母双杂交测定、免疫共沉淀和 GST Pull-down 测定进一步证实。此外，结果表明，MT-1X敲低通过调节FHL3介导的Smad信号传导并通过与FHL3相互作用参与调节G2/M期相关蛋白的表达来促进FHL3诱导的对HepG2细胞的抑制作用。这些发现表明FHL3和MT-1X之间的功能相互作用可能为FHL3调节细胞增殖的机制提供一些线索。
Four and a half LIM domain protein 3 (FHL3) is a member of the FHL protein family that plays roles in the regulation of cell survival, cell adhesion and signal transduction. However, the mechanism of action for FHL3 is not yet clear. The aim of present study was to identify novel binding partner of FHL3 and to explore the underlying mechanism. With the use of yeast two-hybrid screening system, FHL3 was used as the bait to screen human fetal hepatic cDNA library for interacting proteins. Methionine-1X was identified as a novel FHL3 binding partner. The interaction between FHL3 and the full length MT-1X was further confirmed by yeast two-hybrid assay, co-immunoprecipitation and GST pull-down assays. Furthermore,the result demonstrated that MT-1X knockdown promoted the FHL3-induced inhibitory effect on HepG2 cells by regulating FHL3-mediated Smad signaling and involving in the modulation the expression of G2/M phase-related proteins through interaction with FHL3. These findings suggest that functional interactions between FHL3 and MT-1X may provide some clues to the mechanisms of FHL3-regulated cell proliferation.
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影响因子：
5.3
作者：
Dulic, V;Stein, GH;Reed, SI
通讯作者：
Reed, SI
影响因子：
4.8
作者：
Coghill, ID;Brown, S;Mitchell, CA
通讯作者：
Mitchell, CA
DOI：
10.1006/bbrc.1996.1222
发表时间：
1996-08-14
影响因子：
3.1
作者：
Morgan, MJ;Madgwick, AJA
通讯作者：
Madgwick, AJA
影响因子：
5.3
作者：
Fimia, GM;De Cesare, D;Sassone-Corsi, P
通讯作者：
Sassone-Corsi, P
影响因子：
2.9
作者：
KAGI, JHR;SCHAFFER, A
通讯作者：
SCHAFFER, A