Selectivity and affinity of DNA triplex forming oligonucleotides containing the nucleoside analogues 2'-O-methyl-5-(3-amino-1-propynyl)uridine and 2'-O-methyl-5-propynyluridine.
Selectivity and affinity of DNA triplex forming oligonucleotides containing the nucleoside analogues 2'-O-methyl-5-(3-amino-1-propynyl)uridine and 2'-O-methyl-5-propynyluridine.
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DOI：
10.1039/b810709b
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发表时间：
2008-11-21
影响因子：
3.2
通讯作者：
Seidman MM
中科院分区：
文献类型：
--
作者：
Li H;Miller PS;Seidman MM
Triplex forming oligonucleotides (TFOs) containing the nucleoside analogues 2′-O-Methyl-5-propynyluridine (1) and 2′-O-Methyl-5-(3-amino-1-propynyl)uridine (2) were synthesized. The affinity and selectivity of triplex formation by these TFOs were studied by gel shift analysis, Tm value measurement, and association rate assays. The results show that the introduction of 1 and 2 into TFOs can improve the stability of the triplexes under physiological conditions. Optimized distribution of 1 or 2 in the TFOs combined with a cluster of contiguous nucleosides with 2′-aminoethoxy sugars resulted in formation of triplexes with further enhanced stability and improved selectivity.
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影响因子：
3.2
作者：
Mayer, A;Häberli, A;Leumann, CJ
通讯作者：
Leumann, CJ
影响因子：
56.9
作者：
ROBERTS, RW;CROTHERS, DM
通讯作者：
CROTHERS, DM
影响因子：
14.9
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Brazier, JA;Shibata, T;Williams, DM
通讯作者：
Williams, DM
影响因子：
2.9
作者：
Sollogoub, M;Darby, RAJ;Fox, KR
通讯作者：
Fox, KR
影响因子：
2.9
作者：
Lacroix, L;Lacoste, J;Glazer, PM
通讯作者：
Glazer, PM