Structure and functions of powerful transactivators: VP16, MyoD and FoxA.
Structure and functions of powerful transactivators: VP16, MyoD and FoxA.
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DOI：
10.1387/ijdb.103194hh
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Kikyo N
中科院分区：
文献类型：
--
作者：
Hirai H;Tani T;Kikyo N
诱导多能干细胞(IPSC)技术是将一种类型的分化细胞转化为另一种类型的分化细胞的一种很有前途的方法，通过多能状态作为中间步骤。然而，最近的研究表明，直接将一种类型的细胞转化为另一种类型的细胞类型，而不需要经历多能状态。这种直接重新编程的方法依赖于一组高效的转录因子来进行细胞类型转换，可能跳过了更多的生理和多步骤分化过程。试错法通常用于筛选许多候选转录因子，以确定正确的因子组合。然而，我们推测，更好地理解示范性转录激活子的功能机制将有助于识别能够直接重新编程的新因子组合。这篇综述的目的是对三种高效转录激活剂的文献进行批判性的综述：疱疹病毒蛋白VP16，骨骼肌分化的主要调节因子MyoD和肝脏发生的先驱因子FoxA。我们讨论了它们的功能蛋白结构域、相互作用伙伴和染色质重塑机制在基因激活过程中的作用，以了解这些因素是如何打开非激活基因的染色质，并在细胞类型转换过程中重置转录模式的。
Induced pluripotent stem cell (iPSC) technology is a promising approach for converting one type of a differentiated cell into another type of differentiated cell through a pluripotent state as an intermediate step. Recent studies, however, indicate the possibility of directly converting one cell type to another without going through a pluripotent state. This direct reprogramming approach is dependent on a combination of highly potent transcription factors for cell-type conversion, presumably skipping more physiological and multi-step differentiation processes. A trial-and-error strategy is commonly used to screen many candidate transcription factors to identify the correct combination of factors. We speculate, however, that a better understanding of the functional mechanisms of exemplary transcriptional activators will facilitate the identification of novel factor combinations capable of direct reprogramming. The purpose of this review is to critically examine the literature on three highly potent transcriptional activators: the herpes virus protein, VP16; the master regulator of skeletal muscle differentiation, MyoD and the “pioneer” factor for hepatogenesis, FoxA. We discuss the roles of their functional protein domains, interacting partners and chromatin remodeling mechanisms during gene activation to understand how these factors open the chromatin of inactive genes and reset the transcriptional pattern during cell type conversion.
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影响因子：
3.3
作者：
Boxus M;Twizere JC;Legros S;Dewulf JF;Kettmann R;Willems L
通讯作者：
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DOI：
10.1073/pnas.88.13.5675
发表时间：
1991-07-01
影响因子：
11.1
作者：
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16
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64.5
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作者：
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