Out of the dark: transitional subsurface-to-surface microbial diversity in a terrestrial serpentinizing seep (Manleluag, Pangasinan, the Philippines).
Out of the dark: transitional subsurface-to-surface microbial diversity in a terrestrial serpentinizing seep (Manleluag, Pangasinan, the Philippines).
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DOI：
10.3389/fmicb.2015.00044
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发表时间：
2015
影响因子：
5.2
通讯作者：
Arcilla CA
中科院分区：
文献类型：
--
作者：
Woycheese KM;Meyer-Dombard DR;Cardace D;Argayosa AM;Arcilla CA
在赞巴勒蛇绿岩范围内，陆生蛇纹石流体渗透出多种微生物组合。流体属于Ca2+-OH−型水，表明蛇纹岩作用活跃，溶解无机碳（DIC）含量低（<0.5 ppm）。随着离源距离的增加，大气中二氧化碳（CO2）的涌入影响碳酸钙的溶解度，从而引发米级石灰华梯田的形成。在源头和流出通道采集样本，以确定地下微生物群落对地表暴露的反应。提取DNA并提交Illumina MiSeq平台进行高通量16S rRNA基因测序。序列数据的分类鉴定表明，8.1%的总序列读取源与甲烷菌属有关。在源处检测到的其他主要分类包括厌氧分类群，如拟杆菌门（占总序列reads的40.7%）和厚壁菌门（占总序列reads的19.1%）。随着氧化还原电位的增加，噬氢菌的相对丰度增加。在碳酸盐台地，45%的序列序列与减温菌属有关。分类观察和地球化学数据表明，基于最近邻居的能力，几种假定的代谢可能是有利的，包括氢氧化、h2相关的硫循环、甲烷生成、甲烷化、固氮、氨氧化、反硝化、硝酸盐呼吸、甲基化、一氧化碳呼吸和亚铁氧化。扫描电子显微镜和能量色散x射线光谱学表明，微生物活动在渗漏源下游形成的沉淀碳酸盐中产生了化学和物理痕迹。这些数据为未来蛇纹石化渗漏生态系统的研究提供了背景，特别是在热带生物群系方面。
In the Zambales ophiolite range, terrestrial serpentinizing fluid seeps host diverse microbial assemblages. The fluids fall within the profile of Ca2+-OH−-type waters, indicative of active serpentinization, and are low in dissolved inorganic carbon (DIC) (<0.5 ppm). Influx of atmospheric carbon dioxide (CO2) affects the solubility of calcium carbonate as distance from the source increases, triggering the formation of meter-scale travertine terraces. Samples were collected at the source and along the outflow channel to determine subsurface microbial community response to surface exposure. DNA was extracted and submitted for high-throughput 16S rRNA gene sequencing on the Illumina MiSeq platform. Taxonomic assignment of the sequence data indicates that 8.1% of the total sequence reads at the source of the seep affiliate with the genus Methanobacterium. Other major classes detected at the source include anaerobic taxa such as Bacteroidetes (40.7% of total sequence reads) and Firmicutes (19.1% of total reads). Hydrogenophaga spp. increase in relative abundance as redox potential increases. At the carbonate terrace, 45% of sequence reads affiliate with Meiothermus spp. Taxonomic observations and geochemical data suggest that several putative metabolisms may be favorable, including hydrogen oxidation, H2-associated sulfur cycling, methanogenesis, methanotrophy, nitrogen fixation, ammonia oxidation, denitrification, nitrate respiration, methylotrophy, carbon monoxide respiration, and ferrous iron oxidation, based on capabilities of nearest known neighbors. Scanning electron microscopy and energy dispersive X-ray spectroscopy suggest that microbial activity produces chemical and physical traces in the precipitated carbonates forming downstream of the seep's source. These data provide context for future serpentinizing seep ecosystem studies, particularly with regards to tropical biomes.
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影响因子：
4.4
作者：
Brazelton, William J.;Morrill, Penny L.;Schrenk, Matthew O.
通讯作者：
Schrenk, Matthew O.
影响因子：
3.9
作者：
ABRAJANO, TA;STURCHIO, NC;STEVENS, CM
通讯作者：
STEVENS, CM
DOI：
10.1073/pnas.1203849109
发表时间：
2012-10-02
影响因子：
11.1
作者：
Kallmeyer, Jens;Pockalny, Robert;D'Hondt, Steven
通讯作者：
D'Hondt, Steven
影响因子：
5.3
作者：
Etiope, Giuseppe;Schoell, Martin;Hosgormez, Hakan
通讯作者：
Hosgormez, Hakan
DOI：
10.1007/s10295-003-0047-z
发表时间：
2003-04-01
影响因子：
3.4
作者：
Kolari, M;Nuutinen, J;Salkinoja-Salonen, MS
通讯作者：
Salkinoja-Salonen, MS