Mass spectrometric methods for evaluating point mutations.
Mass spectrometric methods for evaluating point mutations.
复制标题
用于评估点突变的质谱方法。
DOI：
10.2144/98241st03
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发表时间：
1998
期刊：
影响因子：
2.7
通讯作者：
Randall W. Nelson
中科院分区：
文献类型：
--
作者：
J. K. Lewis;J. R. Krone;Randall W. Nelson
描述了两种用于部分消化蛋白质的基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱分析的内部校准光谱的方法。蛋白质的部分消化导致大量的离子信号存在于MALDI-TOF质谱中，这又表示分析物中存在的每个氨基酸的显著过采样。这种过采样允许无争议来源的离子信号用作内部校准物，用于评估怀疑含有点突变的片段。与正确的氨基酸序列相关，观察到所有离子信号（校准品和分析物）的质量值落入单个低误差数据集。相反，经验得出的数据应用于不正确的序列将数据分成不同错误的子集。该方法利用了使用MALDI-TOF对蛋白质进行酶促质量作图期间产生的数据的自洽性质，并且它们有助于快速、灵敏和准确地评估蛋白质中存在的点突变。
Two methods for internally calibrating spectra resulting from the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of partially digested proteins are described. Partial digestion of proteins results in a large number of ion signals present in the MALDI-TOF mass spectrum, which in turn represent a significant over-sampling of each amino acid present in the analyte. This over-sampling allows ion signals of undisputed origin to be used as internal calibrants for the evaluation of fragments suspected to contain point mutations. Correlated with the correct amino acid sequence, the mass values of all ion signals (calibrants and analytes) are observed to fall into a single low-error data set. Conversely, empirically derived data applied to an incorrect sequence split the data into subsets of different errors. The methods take advantage of the self-consistent nature of data generated during the enzymatic mass mapping of proteins using MALDI-TOF, and they aid in the rapid, sensitive and accurate evaluation of point mutations present in proteins.
肌红蛋白的正确分子量，肌红蛋白是质谱分析的常用校准物。
DOI：
10.1002/rcm.1290060108
发表时间：
1992
期刊：
Rapid communications in mass spectrometry : RCM
影响因子：
--
作者：
Zaia,J;Annan,RS;Biemann,K
通讯作者：
Biemann,K
影响因子：
7.4
作者：
BEAVIS, RC;CHAIT, BT
通讯作者：
CHAIT, BT