Aequorin luminescence, myosin phosphorylation, and active stress in tracheal smooth muscle.
Aequorin luminescence, myosin phosphorylation, and active stress in tracheal smooth muscle.
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水母发光蛋白发光、肌球蛋白磷酸化和气管平滑肌的主动应激。
DOI：
10.1152/ajpcell.1989.257.6.c1062
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Gunst,SJ
中科院分区：
文献类型：
--
作者：
Gerthoffer,WT;Murphey,KA;Gunst,SJ
在用卡巴胆碱激活犬气管平滑肌的毒蕈碱激活过程中，肌球蛋白磷酸化对外部Ca 2+浓度（[Ca 2 +]o）的敏感性显著高于应激[W. T.格索费尔Am. J.Physiol.250（Cell Physiol.19）：C597-C604，1986]。为了确定细胞内Ca 2+浓度（[Ca 2 +]i）是否与磷酸化或力的变化更密切相关，我们测量了等长力和发光细胞内Ca 2+指示剂水母发光蛋白发出的光，因为在1 μ M卡巴胆碱或60 mM K+存在下[Ca 2 +]o增加。肌球蛋白磷酸化测定使用免疫印迹法在第二组相同处理的肌条。卡巴胆碱刺激增加水母发光蛋白在无钙溶液中孵育的条略有。活性应力和水母发光随后增加平行[Ca 2 +]o的增加。0.05 mM [Ca 2 +]o（0.30 +/- 0.04 mol Pi/mol轻链）下的肌球蛋白磷酸化显著高于无卡巴胆碱的无Ca 2+溶液中的磷酸化（0.12 +/- 0.048 mol Pi/mol轻链），并在1.6 mM [Ca 2 +]o下增加至最大值0.56 +/- 0.03 mol Pi/mol轻链。相反，主动应激和水母发光蛋白在低[Ca 2 +] 0时保持低水平，并在2.4 mM [Ca 2 +] 0时达到最大值。氨甲酰胆碱刺激产生更大的增加肌球蛋白磷酸化和主动应激的水母发光的一个给定的变化比K+去极化。氨甲酰胆碱刺激也产生了不同的磷酸化应力比K+去极化的关系。这些观察结果与卡巴胆碱诱导气管平滑肌收缩蛋白的Ca 2+敏感性增加的可能性一致。
During muscarinic activation of canine tracheal smooth muscle with carbachol, myosin phosphorylation is significantly more sensitive than stress to the external Ca2+ concentration ([Ca2+]o) [W. T. Gerthoffer. Am. J. Physiol. 250 (Cell Physiol. 19): C597-C604, 1986]. To determine whether the intracellular Ca2+ concentration ([Ca2+]i) correlated more closely with changes in phosphorylation or force, we measured isometric force and light emitted by the luminescent intracellular Ca2+ indicator aequorin as [Ca2+]o was increased in the presence of 1 microM carbachol or 60 mM K+. Myosin phosphorylation was measured using an immunoblot assay in a second set of muscle strips treated identically. Stimulation with carbachol increased aequorin luminescence slightly in strips incubated in Ca2+-free solution. Active stress and aequorin luminescence subsequently increased in parallel as [Ca2+]o was increased. Myosin phosphorylation at 0.05 mM [Ca2+]o (0.30 +/- 0.04 mol Pi/mol light chain) was significantly higher than phosphorylation in Ca2+-free solution with no carbachol (0.12 +/- 0.048 mol Pi/mol light chain) and increased to a maximum of 0.56 +/- 0.03 mol Pi/mol light chain at 1.6 mM [Ca2+]o. In contrast, active stress and aequorin luminescence remained low at low [Ca2+]o and reached a maximum at 2.4 mM [Ca2+]o. Stimulation with carbachol produced greater increases in myosin phosphorylation and active stress for a given change in aequorin luminescence than did K+ depolarization. Stimulation with carbachol also produced a different phosphorylation-stress relationship than did K+ depolarization. These observations are consistent with the possibility that carbachol induces increases in the Ca2+ sensitivity of contractile proteins in tracheal smooth muscle.
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气管平滑肌毒蕈碱刺激期间肌球蛋白磷酸化和主动张力的解离。
DOI：
--
发表时间：
1987
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Gerthoffer,WT
通讯作者：
Gerthoffer,WT
影响因子：
3.4
作者：
FINZEL, BC;SALEMME, FR
通讯作者：
SALEMME, FR
雪貂血管扩张剂改变平滑肌细胞质离子钙水平。
DOI：
10.1113/jphysiol.1984.sp015516
发表时间：
1984
期刊：
The Journal of physiology
影响因子：
--
作者：
Morgan,JP;Morgan,KG
通讯作者：
Morgan,KG
通过对单细胞的测量揭示了平滑肌中力和 Ca2 浓度之间的关系。
DOI：
10.1073/pnas.85.11.4109
发表时间：
1988
影响因子：
11.1
作者：
Yagi,S;Becker,PL;Fay,FS
通讯作者：
Fay,FS
肌醇 1,4,5-三磷酸在引发激动剂诱导的狗气管平滑肌收缩中的作用
影响因子：
7.3
作者：
T. Hashimoto;M. Hirata;Y. Ito
通讯作者：
Y. Ito