Age of Rats Affects the Degree of Retinal Neuroinflammatory Response Induced by High Acute Intraocular Pressure.
Age of Rats Affects the Degree of Retinal Neuroinflammatory Response Induced by High Acute Intraocular Pressure.
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大鼠年龄对高眼压所致视网膜神经炎症反应程度的影响
DOI：
10.1155/2022/9404977
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Xia X
中科院分区：
文献类型：
--
作者：
Meng S;Wen D;Xiao J;Zhang Q;Fang W;Xue X;Hu T;Xia X
目的：探讨增龄对急性青光眼大鼠视网膜神经炎性反应的影响。将幼年和老年大鼠随机分为正常对照组、45 毫米汞组、60 毫米汞组和90 毫米汞组。大鼠眼压急剧升高，分别为45 、60 和90 。高眼压治疗3d后，免疫荧光检测视网膜神经节细胞(RGCs)丢失、促炎性小胶质细胞/巨噬细胞和神经毒性星形胶质细胞的形成以及补体C3在视网膜内的沉积。用ELISA法检测视网膜组织中促炎症细胞因子肿瘤坏死因子和白介素1β的蛋白水平。与青年视网膜相比，(1)相同眼压处理下，老年视网膜RGC的丢失更为严重；(2)小胶质细胞/巨噬细胞在老年视网膜对高眼压的反应更倾向于采用促炎表型；(3)高眼压处理更容易导致老年视网膜星形胶质细胞的形成、神经毒性星形胶质细胞的形成和补体C3的沉积；(4)相同眼压处理下，老年视网膜诱导更高水平的促炎细胞因子肿瘤坏死因子和IL-1β。我们的数据表明，衰老影响了高眼压引起的视网膜神经炎性反应的程度，这可能是与年龄相关的RGCs对高眼压的易感性的原因之一。
To investigate whether retinal neuroinflammatory response was affected by aging in a rat model of acute glaucoma. Young adult and aged rats were randomly assigned into normal control, 45 mmHg, 60 mmHg, and 90 mmHg groups. Intraocular pressure (IOP) of rats was acutely elevated to 45 mmHg, 60 mmHg, and 90 mmHg, respectively. Three days after high IOP treatment, loss of retinal ganglion cells (RGCs), formation of proinflammatory microglia/macrophages and neurotoxic astrocytes, and deposition of complement C3 in the retina were detected by immunofluorescence. ELISA was used to assess the protein levels of proinflammatory cytokines TNF and IL-1β in the retina. Compared with young adult retinae, (1) loss of RGCs was more severe in aged retinae under the same IOP treatment, (2) microglia/macrophages were more prone to adopt proinflammatory phenotype in aged retinae in response to elevated IOP, (3) high IOP treatment induced astrogliosis, formation of neurotoxic astrocytes, and deposition of complement C3 more easily in aged retinae, and (4) aged retinae induced higher levels of proinflammatory cytokines TNF and IL-1β under the same IOP treatment. Our data indicated that aging affects the degree of retinal neuroinflammatory response initiated by ocular hypertension, which may contribute to the age-related susceptibility of RGCs to elevated IOP.
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影响因子：
25
作者：
Habib, Naomi;McCabe, Cristin;Medina, Sedi;Varshavsky, Miriam;Kitsberg, Daniel;Dvir-Szternfeld, Raz;Green, Gilad;Dionne, Danielle;Nguyen, Lan;Marshall, Jamie L.;Chen, Fei;Zhang, Feng;Kaplan, Tommy;Regev, Aviv;Schwartz, Michal
通讯作者：
Schwartz, Michal
影响因子：
4.4
作者：
Abdul, Yasir;Akhter, Naseem;Husain, Shahid
通讯作者：
Husain, Shahid
Act-MMP3 的区域表达导致急性视网膜缺血/再灌注损伤后神经节细胞层神经元的选择性丢失
影响因子：
2
作者：
Hu, Tu;Wang, Shuchao;Huang, Jufang
通讯作者：
Huang, Jufang
影响因子：
4.6
作者：
Madeira MH;Boia R;Santos PF;Ambrósio AF;Santiago AR
通讯作者：
Santiago AR
影响因子：
15.1
作者：
Haage, Verena;Elmadany, Nirmeen;Kettenmann, Helmut
通讯作者：
Kettenmann, Helmut