Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering.
Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering.
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DOI：
10.1186/s12951-020-00732-0
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发表时间：
2020-11-23
影响因子：
10.2
通讯作者：
Alaminos M
中科院分区：
文献类型：
--
作者：
Chato-Astrain J;Chato-Astrain I;Sánchez-Porras D;García-García ÓD;Bermejo-Casares F;Vairo C;Villar-Vidal M;Gainza G;Villullas S;Oruezabal RI;Ponce-Polo Á;Garzón I;Carriel V;Campos F;Alaminos M
严重烧伤患者的治疗具有挑战性，特别是由于假单胞菌感染的高风险。在目前的工作中，我们已经产生了一种新的模型的生物人工真皮替代组织工程治疗感染伤口使用纤维蛋白-琼脂糖生物材料功能化与纳米结构的脂质载体（NLC）负载两种抗假单胞菌抗生素：多粘菌素E甲磺酸钠（SCM）和阿米卡星（AMK）。结果表明，新型组织样替代物对假单胞菌培养物具有强的抗菌作用，与NLC浓度成正比。在功能化真皮替代物中的游离DNA定量、WST-1和Caspase 7免疫组织化学测定表明，在大多数研究组中，功能化既不影响细胞活力，也不影响细胞增殖。此外，PCNA和KI 67的免疫组织化学和胶原蛋白和蛋白多糖的组织化学显示，细胞增殖，并在功能化组织中代谢活跃，与对照组没有差异。当功能化组织的生物力学特征，我们发现，NLC能够改善这些人工组织的一些主要的生物力学性能，虽然这在很大程度上取决于NLC的类型和浓度。这些结果表明，纤维蛋白-琼脂糖人真皮替代物与负载NLC的抗菌剂的功能化是能够提高这些替代物的抗菌和生物力学性能，没有检测到的副作用。这为功能化组织的未来临床应用打开了大门。
Treatment of patients affected by severe burns is challenging, especially due to the high risk of Pseudomonas infection. In the present work, we have generated a novel model of bioartificial human dermis substitute by tissue engineering to treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded with two anti-Pseudomonas antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results show that the novel tissue-like substitutes have strong antibacterial effect on Pseudomonas cultures, directly proportional to the NLC concentration. Free DNA quantification, WST-1 and Caspase 7 immunohistochemical assays in the functionalized dermis substitute demonstrated that neither cell viability nor cell proliferation were affected by functionalization in most study groups. Furthermore, immunohistochemistry for PCNA and KI67 and histochemistry for collagen and proteoglycans revealed that cells proliferated and were metabolically active in the functionalized tissue with no differences with controls. When functionalized tissues were biomechanically characterized, we found that NLCs were able to improve some of the major biomechanical properties of these artificial tissues, although this strongly depended on the type and concentration of NLCs. These results suggest that functionalization of fibrin-agarose human dermal substitutes with antibiotic-loaded NLCs is able to improve the antibacterial and biomechanical properties of these substitutes with no detectable side effects. This opens the door to future clinical use of functionalized tissues.
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影响因子：
3.7
作者：
Oliveira AC;Garzón I;Ionescu AM;Carriel V;Cardona Jde L;González-Andrades M;Pérez Mdel M;Alaminos M;Campos A
通讯作者：
Campos A
影响因子：
5.3
作者：
Chato-Astrain, Jesus;Campos, Fernando;Carriel, Victor
通讯作者：
Carriel, Victor
影响因子：
3
作者：
Fuste, Ester;Lopez-Jimenez, Lidia;Vinas, Miguel
通讯作者：
Vinas, Miguel
影响因子：
3.1
作者：
Martin-Piedra, M. A.;Alfonso-Rodriguez, C. A.;Garzon, I
通讯作者：
Garzon, I
影响因子：
4
作者：
Campos, Fernando;Bonhome-Espinosa, Ana B.;Carriel, Victor
通讯作者：
Carriel, Victor