RNA-Seq analysis of differential gene expression in Betula luminifera xylem during the early stages of tension wood formation.
RNA-Seq analysis of differential gene expression in Betula luminifera xylem during the early stages of tension wood formation.
复制标题
RNA-Seq分析光桦木质部张力木形成早期差异基因表达
作者：
Cai M;Huang H;Ni F;Tong Z;Lin E;Zhu M
光皮桦温克勒是我国南方广泛分布的重要经济阔叶树种。然而，B的基因组信息很少。光壳属（luminifera）是已知的，但关于该种木材形成的分子机制知之甚少。同时，很少有人致力于研究木本植物张力木材形成过程中的早期转录变化。首先生成包含45，700个Unigenes的参考转录组数据集，并且通过针对四个公共数据库的BLAST相似性搜索注释了35，135个（76.9%）Unigenes。在此基础上，分析了受拉材形成早期基因表达的变化。基因表达谱分析表明，在张力木材形成的早期阶段，共有13，273个Unigenes受到差异调节。纤维素和木质素生物合成中的大多数基因被强调，以揭示其在张力木材形成的生物学意义。此外，许多参与生长素反应途径的基因的转录水平在张力木材形成的早期阶段显着变化。此外，还鉴定了18个与纤维素合成关键酶共表达的TF。我们的研究结果揭示了与TW形成相关的转录变化，并确定了调控这一过程的潜在关键基因。这些结果将有助于剖析木材形成的分子机理，并为B的分子标记辅助选择提供关键候选基因。光叶类。
Betula luminifera H. Winkler, which is widely distributed in southern China, is an economically important broadleaf tree species. However, little genomic information of B. luminifera is available, and little is known about the molecular mechanisms of wood formation in this species. Meanwhile, few efforts have focused on investigating the early transcriptional changes during tension wood formation in woody plants. A reference transcriptome dataset was first generated containing 45,700 Unigenes, and 35,135 (76.9%) Unigenes were annotated by a BLAST similarity search against four public databases. Then, based on an anatomical investigation, the global gene expression changes during the early stages of tension wood formation were analyzed. Gene expression profiling showed that a total of 13,273 Unigenes were differentially regulated during the early stages of tension wood formation. Most genes involved in cellulose and lignin biosynthesis were highlighted to reveal their biological importance in tension wood formation. In addition, the transcription levels of many genes involved in the auxin response pathway were significantly changed during the early stages of tension wood formation. Furthermore, 18 TFs co-expressed with key enzymes of cellulose synthesis were identified. Our results revealed the transcriptional changes associated with TW formation and identified potential key genes in the regulation of this process. These results will help to dissect the molecular mechanism of wood formation and provide key candidate genes for marker-assisted selection in B. luminifera.
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影响因子：
4.4
作者：
Feng C;Chen M;Xu CJ;Bai L;Yin XR;Li X;Allan AC;Ferguson IB;Chen KS
通讯作者：
Chen KS
影响因子：
4.3
作者：
Bhandari, Suchita;Fujino, Takeshi;Joshi, Chandrashekhar P.
通讯作者：
Joshi, Chandrashekhar P.
DOI：
10.1073/pnas.172520999
发表时间：
2002-10-15
影响因子：
11.1
作者：
Cong, B;Liu, JP;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
4.4
作者：
Huang HH;Xu LL;Tong ZK;Lin EP;Liu QP;Cheng LJ;Zhu MY
通讯作者：
Zhu MY
DOI：
10.1073/pnas.0501181102
发表时间：
2005-03-29
影响因子：
11.1
作者：
Hackbusch, J;Richter, K;Uhrig, JF
通讯作者：
Uhrig, JF