Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance.
Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance.
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DOI：
10.1016/j.bpj.2014.01.008
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发表时间：
2014-02-18
影响因子：
3.4
通讯作者：
Schiemann, Olav
中科院分区：
文献类型：
--
作者：
Ward, Richard;Pliotas, Christos;Branigan, Emma;Hacker, Christian;Rasmussen, Akiko;Hagelueken, Gregor;Booth, Ian R.;Miller, Samantha;Lucocq, John;Naismith, James H.;Schiemann, Olav
机械敏感通道蛋白是细菌抵抗渗透休克的重要安全阀，在高等生物中参与触觉和声波的感知。小电导的机械敏感通道（MscS）已被广泛研究。脉冲电子-电子双共振（PELDOR或DEER）证实，从晶体结构中可以看出，跨膜螺旋的外环不会与形成孔的螺旋堆积在一起，从而产生明显的空隙。这种空洞与双层间充质干细胞的功能形式的相关性是争论的主题。在这里，我们报告了PELDOR测量的MscS重组成两个脂质双分子层系统：纳米圆盘和单胞体。来自PELDOR数据的多个突变体的距离测量与蛋白质的洗涤剂溶液排列一致。因此，我们得出结论，跨膜螺旋的相对位置在细胞双层的模拟中被保留下来，并且明显的空隙不是洗涤剂溶液的人造产物，而是蛋白质的一种特性，在任何分子门控机制中都必须考虑到这一点。
Mechanosensitive channel proteins are important safety valves against osmotic shock in bacteria, and are involved in sensing touch and sound waves in higher organisms. The mechanosensitive channel of small conductance (MscS) has been extensively studied. Pulsed electron-electron double resonance (PELDOR or DEER) of detergent-solubilized protein confirms that as seen in the crystal structure, the outer ring of transmembrane helices do not pack against the pore-forming helices, creating an apparent void. The relevance of this void to the functional form of MscS in the bilayer is the subject of debate. Here, we report PELDOR measurements of MscS reconstituted into two lipid bilayer systems: nanodiscs and bicelles. The distance measurements from multiple mutants derived from the PELDOR data are consistent with the detergent-solution arrangement of the protein. We conclude, therefore, that the relative positioning of the transmembrane helices is preserved in mimics of the cell bilayer, and that the apparent voids are not an artifact of detergent solution but a property of the protein that will have to be accounted for in any molecular mechanism of gating.
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旋转对称的同性恋者中的Peldor。
影响因子：
1.7
作者：
Giannoulis A;Ward R;Branigan E;Naismith JH;Bode BE
通讯作者：
Bode BE
影响因子：
16.8
作者：
Hanelt, Inga;Wunnicke, Dorith;Slotboom, Dirk Jan
通讯作者：
Slotboom, Dirk Jan
影响因子：
3.3
作者：
Jeschke, Gunnar;Sajid, Muhammad;Godt, Adelheid
通讯作者：
Godt, Adelheid
影响因子：
15
作者：
Dalmas O;Hyde HC;Hulse RE;Perozo E
通讯作者：
Perozo E
影响因子：
5.6
作者：
Klare, JP;Bordignon, E;Engelhard, M
通讯作者：
Engelhard, M