Polarized targeting of L1-CAM regulates axonal and dendritic bundling in vitro.
Polarized targeting of L1-CAM regulates axonal and dendritic bundling in vitro.
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DOI：
10.1111/j.1460-9568.2010.07447.x
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Gu C
中科院分区：
文献类型：
--
作者：
Barry J;Gu Y;Gu C
正确的轴突和树突束是必要的建立神经元连接和突触输入的同步，分别。L1-CAM家族的细胞粘附分子调节轴突导向和成束、神经元迁移、树突形态和突触可塑性。这些分子如何发挥如此多的不同作用仍不清楚。在这里，我们表明，极化轴突树突靶向的鸟类L1-CAM蛋白，NgCAM，可以调节开关捆绑的两个主要车厢的神经元。使用一种新的体外模型来研究神经突-神经突相互作用，我们发现表达的轴突NgCAM通过免疫球蛋白（IG）结构域的反式嗜同性相互作用诱导强大的轴突集束。有趣的是，树突状集束是由NgCAM的树突状靶向诱导的，这是通过删除其纤连蛋白（FN）重复序列或阻断蛋白激酶的活性引起的。与NgCAM结果一致，小鼠L1 CAM的表达也诱导轴突集束，阻断激酶活性破坏其轴突靶向。此外，反式嗜同性相互作用稳定了束形成，可能是通过将NgCAM蛋白募集到接触位点并促进引导轴突生长。总而言之，我们的结果表明L1-CAM的精确定位对于在神经回路中建立适当的细胞与细胞接触非常重要。
Proper axonal and dendritic bundling is essential for the establishment of neuronal connections and the synchronization of synaptic inputs, respectively. Cell adhesion molecules of the L1-CAM family regulate axon guidance and fasciculation, neuron migration, dendrite morphology, and synaptic plasticity. How these molecules play so many different roles remains unclear. Here we show that polarized axon-dendrite targeting of an avian L1-CAM protein, NgCAM, can regulate the switch of bundling of the two major compartments of neurons. Using a new in vitro model for studying neurite-neurite interactions, we found that expressed axonal NgCAM induced robust axonal bundling via the trans-homophilic interaction of immunoglobulin (Ig) domains. Interestingly, dendritic bundling was induced by the dendritic targeting of NgCAM, caused by either deleting its fibronectin (FN) repeats or blocking activities of protein kinases. Consistent with the NgCAM results, expression of mouse L1CAM also induced axonal bundling and blocking kinase activities disrupted its axonal targeting. Furthermore, the trans-homophilic interaction stabilized the bundle formation, likely through recruiting NgCAM proteins to contact sites and promoting guided axon outgrowth. Taken together, our results suggest that precise localization of L1-CAM is important for establishing proper cell-cell contacts in neural circuits.
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影响因子：
4.6
作者：
Bak, M;Fraser, SE
通讯作者：
Fraser, SE
影响因子：
5.3
作者：
Demyanenko, GP;Maness, PF
通讯作者：
Maness, PF
影响因子：
4.8
作者：
HORTSCH, M;WANG, YME;BIEBER, AJ
通讯作者：
BIEBER, AJ
DOI：
10.1083/jcb.200111076
发表时间：
2002-06-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dickson TC;Mintz CD;Benson DL;Salton SR
通讯作者：
Salton SR
影响因子：
3.3
作者：
ESCOBAR, MI;PIMIENTA, H;KOSIK, KS
通讯作者：
KOSIK, KS