The replication of a mouse adapted SARS-CoV in a mouse cell line stably expressing the murine SARS-CoV receptor mACE2 efficiently induces the expression of proinflammatory cytokines.
The replication of a mouse adapted SARS-CoV in a mouse cell line stably expressing the murine SARS-CoV receptor mACE2 efficiently induces the expression of proinflammatory cytokines.
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DOI：
10.1016/j.jviromet.2013.07.039
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发表时间：
2013-11
影响因子：
3.1
通讯作者：
DeDiego ML
中科院分区：
文献类型：
--
作者：
Regla-Nava JA;Jimenez-Guardeño JM;Nieto-Torres JL;Gallagher TM;Enjuanes L;DeDiego ML
已经产生了稳定表达小鼠血管紧张素转换酶2 （mACE2）的延迟性脑肿瘤（DBT）小鼠细胞系。这些细胞系对小鼠适应的sars冠状病毒感染高度敏感。SARS-CoV-MA15有效诱导DBT-mACE2细胞中促炎因子和IFN-β的表达。DBT-mACE2细胞为评估sars - cov -宿主相互作用研究提供了一个良好的实验系统，该系统与体内系统具有物种同源性。小鼠适应（MA15）严重急性呼吸综合征（SARS）冠状病毒（CoV）感染常规小鼠可再现人类SARS的许多方面，如肺部病理改变、病毒血症、嗜中性粒细胞和致死率。然而，目前还没有建立起对小鼠适应性sars冠状病毒感染高度敏感的小鼠细胞系。在这项工作中，已经产生了高效可转染的小鼠细胞系，稳定表达小鼠SARS-CoV受体血管紧张素转换酶2 （ACE2）。这些细胞产生了高的SARS-CoV-MA15滴度，也作为空斑测定的优秀工具。此外，在这些细胞系中，SARS- cov - ma15诱导了促炎细胞因子和IFN-β的表达，这与在感染SARS- cov和SARS患者的实验动物模型中观察到的情况相似。这些细胞系是在小鼠细胞系统中进行体外研究的宝贵工具，反映了用于体内研究SARS-CoV-MA15发病机制的物种。
Delayed brain tumor (DBT) mouse cell lines stably expressing the murine angiotensin converting enzyme 2 (mACE2) have been generated. The cell lines are highly susceptible to mouse-adapted SARS-CoV infection. SARS-CoV-MA15 efficiently induced the expression of proinflammatory cytokines and IFN-β in DBT-mACE2 cells. DBT-mACE2 cells provide a good experimental system that is species-homologous to the in vivo systems for evaluating SARS-CoV-host interaction studies. Infection of conventional mice with a mouse adapted (MA15) severe acute respiratory syndrome (SARS) coronavirus (CoV) reproduces many aspects of human SARS such as pathological changes in lung, viremia, neutrophilia, and lethality. However, established mouse cell lines highly susceptible to mouse-adapted SARS-CoV infection are not available. In this work, efficiently transfectable mouse cell lines stably expressing the murine SARS-CoV receptor angiotensin converting enzyme 2 (ACE2) have been generated. These cells yielded high SARS-CoV-MA15 titers and also served as excellent tools for plaque assays. In addition, in these cell lines, SARS-CoV-MA15 induced the expression of proinflammatory cytokines and IFN-β, mimicking what has been observed in experimental animal models infected with SARS-CoV and SARS patients. These cell lines are valuable tools to perform in vitro studies in a mouse cell system that reflects the species used for in vivo studies of SARS-CoV-MA15 pathogenesis.
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DOI：
10.1016/s0140-6736(03)13967-0
发表时间：
2003-07-26
期刊：
Lancet (London, England)
影响因子：
--
作者：
Kuiken T;Fouchier RA;Schutten M;Rimmelzwaan GF;van Amerongen G;van Riel D;Laman JD;de Jong T;van Doornum G;Lim W;Ling AE;Chan PK;Tam JS;Zambon MC;Gopal R;Drosten C;van der Werf S;Escriou N;Manuguerra JC;Stöhr K;Peiris JS;Osterhaus AD
通讯作者：
Osterhaus AD
影响因子：
5.4
作者：
Gosert, R;Kanjanahaluethai, A;Baker, SC
通讯作者：
Baker, SC
影响因子：
2.7
作者：
Hattermann, K;Müller, MA;Niedrig, M
通讯作者：
Niedrig, M
影响因子：
5.4
作者：
Kopecky-Bromberg, Sarah A.;Martinez-Sobrido, Luis;Palese, Peter
通讯作者：
Palese, Peter
影响因子：
5
作者：
Frieman, Matthew;Heise, Mark;Baric, Ralph
通讯作者：
Baric, Ralph