BIOME-Preserve: A novel storage and transport medium for preserving anaerobic microbiota samples for culture recovery.
BIOME-Preserve: A novel storage and transport medium for preserving anaerobic microbiota samples for culture recovery.
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DOI：
10.1371/journal.pone.0261820
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Cox S
中科院分区：
文献类型：
--
作者：
Hyde ER;Lozano H;Cox S
用于研究人类微生物组的基于测序的方案已经发现了大量关于微生物组与人类健康之间关系的信息。但是，如果没有基于培养的研究来表型感兴趣的物种并建立用于动物模型的培养物收集，这些微生物就不能被用作治疗靶点。传统的样品收集方案集中于保存核酸和代谢物，并且在很大程度上不适合保存敏感的厌氧菌以用于以后的培养物回收。在这里，我们介绍了一种新的微生物组保存试剂盒（BIOME-Preserve），它有助于从人类粪便中回收厌氧菌。使用培养物回收和浅层全基因组鸟枪测序的组合，我们表征了从新鲜人粪便和在室温下在BIOME-Preserve中保存长达120小时的人粪便中培养的厌氧菌。我们回收了微生物组研究人员感兴趣的几个物种，包括双歧杆菌属，拟杆菌属，布劳特氏菌属，霍氏真杆菌（现为霍氏厌氧丁酸菌）、嗜粘蛋白阿克曼氏菌和普氏粪杆菌。我们还证明，在-80 ° C下冷冻不会对我们从BIOME-Preserve培养生物体的能力产生不利影响，这表明它既适合作为运输培养基，也适合作为长期超低温储存的培养基。总之，我们的研究结果表明，BIOME-Preserve对于从人体样本中收集、运输和培养厌氧菌是实用的，可以帮助研究人员更好地了解微生物组与人类健康之间的联系，以及如何通过基于微生物组的新疗法来利用这种联系。
Sequencing-based protocols for studying the human microbiome have unearthed a wealth of information about the relationship between the microbiome and human health. But these microbes cannot be leveraged as therapeutic targets without culture-based studies to phenotype species of interest and to establish culture collections for use in animal models. Traditional sample collection protocols are focused on preserving nucleic acids and metabolites and are largely inappropriate for preserving sensitive anaerobic bacteria for later culture recovery. Here we introduce a novel microbiome preservation kit (BIOME-Preserve) that facilitates recovery of anaerobic bacteria from human stool. Using a combination of culture recovery and shallow whole-genome shotgun sequencing, we characterized the anaerobes cultured from fresh human stool and from human stool held at room temperature in BIOME-Preserve for up to 120 hours. We recovered several species of interest to microbiome researchers, including Bifidobacterium spp., Bacteroides spp., Blautia spp., Eubacterium halii (now Anaerobutyricum hallii), Akkermansia muciniphila, and Faecalibacterium prausnitzii. We also demonstrated that freezing at -80°C did not adversely affect our ability to culture organisms from BIOME-Preserve, suggesting that it is appropriate both as a transport medium and as a medium for longer-term ultra-cold storage. Together, our results suggest BIOME-Preserve is practical for the collection, transport, and culture of anaerobic bacteria from human samples and can help enable researchers to better understand the link between the microbiome and human health and how to leverage that link through novel microbiome-based therapeutics.
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DOI：
10.1128/aem.18.5.723-727.1969
发表时间：
1969-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
LOESCHE, WJ
通讯作者：
LOESCHE, WJ
影响因子：
6.4
作者：
Benitez-Paez, Alfonso;Gomez del Pugar, Eva M.;Sanz, Yolanda
通讯作者：
Sanz, Yolanda
影响因子：
9.4
作者：
TALLY, FP;STEWART, PR;ROSENBLATT, JE
通讯作者：
ROSENBLATT, JE
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.3
作者：
Vandeputte D;Tito RY;Vanleeuwen R;Falony G;Raes J
通讯作者：
Raes J