Assays for Membrane Complement Receptors

Assays for Membrane Complement Receptors
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膜补体受体的测定

DOI:
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发表时间:
1994
影响因子:
--
通讯作者:
G. D. Ross
G. D. Ross
中科院分区:
--
文献类型:
--
作者:
Lynda D. O'Rear;G. D. Ross

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白细胞为补体(C)系统的活化产物表达几种类型的受体。本单元描述了这些受体的一个子集的检测:那些针对C3固定片段(CR1, CR2, CR3和CR4)的特异性检测。虽然这些试验是为人类C受体的测试而设计的,但它们通常可以用啮齿类动物(例如小鼠、大鼠或豚鼠)细胞代替啮齿类动物特异性试剂进行。提出了两种基本方案,用于在贴壁和非贴壁细胞上进行CR1、CR2、CR3和CR4的玫瑰花结试验。分离的白细胞可以在悬浮液中或附着在表面上检测玫瑰结的形成。非贴壁细胞,如淋巴细胞、NK细胞或来自未分化的白细胞细胞系的细胞必须在悬液中进行测试。该装置还包含三个支持方案,用于绵羊红细胞- C3复合物的形成和定量,用于两个基本方案。
Leukocytes express several types of receptors for activated products of the complement (C) system. This unit describes assays for a subset of these receptors: those specific for fixed fragments of C3 (CR1, CR2, CR3, and CR4). Although the assays have been designed for tests of human C receptors, they can often be carried out with rodent (e.g., mouse, rat, or guinea pig) cells by substitution of rodent‐specific reagents. Two basic protocols are presented for rosette assay of CR1, CR2, CR3, and CR4 on adherent and nonadherent cells. Isolated leukocytes may be tested for rosette formation in suspension or adherent to a surface. Nonadherent cells such as lymphocytes, NK cells, or cells from an undifferentiated leukocyte cell line must be tested in suspension. The unit also contains three support protocols for formation and quantitation of sheep erythrocyte‐C3 complexes for use in the two basic protocols.
DOI: 10.4049/jimmunol.135.3.2005
发表时间: 1985-09
影响因子: 4.4
作者:
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CR2、Epstein-Barr 病毒的 B 淋巴细胞受体和 C3d 补体片段的免疫生物学。
DOI: 10.1146/annurev.iy.06.040188.000505
发表时间: 1988
影响因子: 29.7
作者:
Cooper,NR;Moore,MD;Nemerow,GR
通讯作者: Nemerow,GR
CD11/CD18 白细胞糖蛋白缺乏症。
DOI: --
发表时间: 1988
期刊: Hematology/oncology clinics of North America
影响因子: --
作者:
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通讯作者: Freyer,DR
DOI: 10.1172/jci113643
发表时间: 1988-08-01
影响因子: 15.9
作者:
MYONES, BL;DALZELL, JG;ROSS, GD
通讯作者: ROSS, GD
DOI: 10.1073/pnas.81.3.881
发表时间: 1984-02
影响因子: 11.1
作者:
J. Weis;T. Tedder;D. Fearon
通讯作者: J. Weis;T. Tedder;D. Fearon