Characterization of the humoral immune response to glutamic acid decarboxylase in patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) and/or type 1 diabetes.
Characterization of the humoral immune response to glutamic acid decarboxylase in patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) and/or type 1 diabetes.
复制标题
自身免疫性多内分泌病-念珠菌病-外胚层营养不良 (APECED) 和/或 1 型糖尿病患者对谷氨酸脱羧酶的体液免疫反应的特征。
DOI：
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复制
发表时间：
2005
影响因子：
5.8
通讯作者：
M. Knip
中科院分区：
文献类型：
--
作者：
M. Ronkainen;T. Härkönen;J. Perheentupa;M. Knip
客观化
对谷氨酸脱羧酶(GAD65)的体液自身免疫反应在1型糖尿病患者和自身免疫性多内分泌病-念珠菌病-外胚层营养不良(APECED)综合征中都很常见，而显性1型糖尿病在APECED患者中相对较少被诊断出来。这项研究的目的是评估1型糖尿病发病率的这种差异是否与对GAD65的体液免疫反应的变异性有关，GAD65是1型糖尿病的主要自身抗原之一。
方法
用放射结合试验分析了20例APECED患者和20例新诊断的1型糖尿病患者的GAD65抗原表位和同型自身抗体。
结果
在APECED患者中，GAD65自身抗体主要针对GAD65的中间和羧基末端区域，偶尔也包括氨基末端区域，主要由IgG1亚类组成，少数为IgG2和IgG4亚类。在1型糖尿病和APECED患者中，表位特异性和同型特异性GAD65自身抗体的分布相似，只是在1型糖尿病患者中观察到的IgG2亚类抗体更多且水平更高(P<；0.05)。这些测量参数都没有区分患有1型糖尿病的APECED患者和没有1型糖尿病的APECED患者。
结论
APECED相关GAD65的体液自身免疫与1型糖尿病观察到的没有显著差异；只有IgG2-GAD65抗体可能与后者更密切相关。
OBJECTIVE
A humoral autoimmune response to glutamic acid decarboxylase (GAD65) is common both in patients with type 1 diabetes and in those with the autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) syndrome, while overt type 1 diabetes is relatively rarely diagnosed in APECED patients. The aim of this study was to assess whether this difference in the incidence of type 1 diabetes is associated with variability in the humoral immune response to GAD65, one of the major autoantigens in type 1 diabetes.
METHODS
Epitope- and isotype-specific GAD65 autoantibodies were analysed in 20 patients with APECED and 20 patients with newly diagnosed type 1 diabetes alone by radiobinding assays.
RESULTS
GAD65 autoantibodies targeted the middle and carboxy-terminal regions of GAD65 and occasionally the amino-terminal region in the APECED patients and comprised mainly the IgG1 subclass and less frequently the IgG2 and IgG4 subclasses. The profile of epitope- and isotype-specific GAD65 autoantibodies was similar in type 1 diabetes and APECED, except that IgG2 subclass antibodies were observed more often and at higher levels in the patients with type 1 diabetes alone (P < 0.05). None of the measured parameters separated APECED patients with type 1 diabetes from those without type 1 diabetes.
CONCLUSION
APECED-associated humoral autoimmunity to GAD65 does not differ markedly from that observed in type 1 diabetes; only IgG2-GAD65 antibodies may be more closely associated with the latter entity.
DOI：
10.1073/pnas.89.6.2115
发表时间：
1992-03-15
影响因子：
11.1
作者：
BU, DF;ERLANDER, MG;TOBIN, AJ
通讯作者：
TOBIN, AJ
自身免疫性多内分泌综合征 I 型患者的谷氨酸脱羧酶抗体和胰岛素依赖型糖尿病。
DOI：
10.1210/jcem.81.4.8636356
发表时间：
1996
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Tuomi,T;Björses,P;Falorni,A;Partanen,J;Perheentupa,J;Lernmark,A;Miettinen,A
通讯作者：
Miettinen,A